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Second derivative spectroscopy has been used to resolve the complex protein spectrum in the near-ultraviolet region and the contributions of the three aromatic chromophores have been evaluated. A method for the direct quantitative determination of phenylalanine and tryptophan in proteins has been carried out. Phenylalanine determination has been carried out in the spectral region between 250 and 265 nm, where there are no significant contributions from other aromatic chromophores. Tryptophan determination has been performed in the 290-295-nm region and the experimental values have been corrected for the presence of tyrosine. The results obtained on 10 highly purified proteins have been found in good agreement with those obtained from sequence analysis.
C Balestrieri, and
G Colonna, and
A Giovane, and
G Irace, and
L Servillo
December 1979,
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C Balestrieri, and
G Colonna, and
A Giovane, and
G Irace, and
L Servillo
January 1980,
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C Balestrieri, and
G Colonna, and
A Giovane, and
G Irace, and
L Servillo
February 2008,
European biophysics journal : EBJ,
C Balestrieri, and
G Colonna, and
A Giovane, and
G Irace, and
L Servillo
November 1994,
Biokhimiia (Moscow, Russia),
C Balestrieri, and
G Colonna, and
A Giovane, and
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L Servillo
May 1982,
Biochemistry,
C Balestrieri, and
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A Giovane, and
G Irace, and
L Servillo
March 1994,
Bioorganicheskaia khimiia,
C Balestrieri, and
G Colonna, and
A Giovane, and
G Irace, and
L Servillo
September 1990,
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C Balestrieri, and
G Colonna, and
A Giovane, and
G Irace, and
L Servillo
September 1982,
Analytical biochemistry,
C Balestrieri, and
G Colonna, and
A Giovane, and
G Irace, and
L Servillo
December 1956,
Klinische Wochenschrift,
C Balestrieri, and
G Colonna, and
A Giovane, and
G Irace, and
L Servillo
January 1958,
Biokhimiia (Moscow, Russia),
