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A combination of immunocytochemistry at light and electron microscopic levels, direct radioimmunoassay and measurement after gel chromatography have been used to identify and characterise a glucagon-like peptide detected in human foetal stomach. Immunocytochemistry, with region specific antisera, demonstrated that the glucagon-containing cells were indistinguishable from pancreatic A cells. Radioimmunoassay of tissue extracts confirmed the presence of significant quantities of glucagon, mean 21 pmol/g wet weight (range 14-29) in 16-26 week old foetuses, increasing to 41 pmol/g wet weight (range 31-52) in 33-30 week old foetuses and after gel chromatography the peptide was found to elute at the same position as standard porcine glucagon. It is apparent, therefore, that the human foetal fundus contains significant quantities of true pancreatic-type glucagon.
A M Buchan, and
M G Bryant, and
B A Stein, and
M Gregor, and
M A Ghatei, and
J F Morris, and
S R Bloom, and
J M Polak
August 1984,
The Histochemical journal,
A M Buchan, and
M G Bryant, and
B A Stein, and
M Gregor, and
M A Ghatei, and
J F Morris, and
S R Bloom, and
J M Polak
November 1971,
The Journal of endocrinology,
A M Buchan, and
M G Bryant, and
B A Stein, and
M Gregor, and
M A Ghatei, and
J F Morris, and
S R Bloom, and
J M Polak
January 1972,
Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove,
A M Buchan, and
M G Bryant, and
B A Stein, and
M Gregor, and
M A Ghatei, and
J F Morris, and
S R Bloom, and
J M Polak
November 1976,
Histochemistry,
A M Buchan, and
M G Bryant, and
B A Stein, and
M Gregor, and
M A Ghatei, and
J F Morris, and
S R Bloom, and
J M Polak
January 1972,
Acta diabetologica latina,
A M Buchan, and
M G Bryant, and
B A Stein, and
M Gregor, and
M A Ghatei, and
J F Morris, and
S R Bloom, and
J M Polak
January 1983,
International journal of obesity,
A M Buchan, and
M G Bryant, and
B A Stein, and
M Gregor, and
M A Ghatei, and
J F Morris, and
S R Bloom, and
J M Polak
October 1981,
Diabetes,
A M Buchan, and
M G Bryant, and
B A Stein, and
M Gregor, and
M A Ghatei, and
J F Morris, and
S R Bloom, and
J M Polak
January 1980,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
A M Buchan, and
M G Bryant, and
B A Stein, and
M Gregor, and
M A Ghatei, and
J F Morris, and
S R Bloom, and
J M Polak
March 1998,
Transplantation proceedings,
A M Buchan, and
M G Bryant, and
B A Stein, and
M Gregor, and
M A Ghatei, and
J F Morris, and
S R Bloom, and
J M Polak
January 1977,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
