Biomaterial Database

Enzymatic methylation of the membrane-bound nicotinic acetylcholine receptor. 1982

D D Flynn, and Y Kloog, and L T Potter, and J Axelrod

The acetylcholine receptor of the Torpedo electric organ acts as substrate for the enzyme protein carboxyl methyltransferase within receptor-enriched membranes and after it is purified. In right-side-out receptor-enriched vesicles, protein of the membrane. All four receptor subunits were found to be methylated from the inside as well outside of the membrane vesicles. The higher molecular weight subunits were found to be methylated to a greater degree than the lower molecular weight subunits.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008745	Methylation	Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)	Methylations
D011496	Protein Methyltransferases	Enzymes that catalyze the methylation of amino acids after their incorporation into a polypeptide chain. S-Adenosyl-L-methionine acts as the methylating agent. EC 2.1.1.	Protein Methylase,Protein Methylases,Protein Methyltransferase,Methylase, Protein,Methylases, Protein,Methyltransferase, Protein,Methyltransferases, Protein
D011497	Protein O-Methyltransferase	An enzyme that catalyzes the transfer of methyl groups from S-adenosylmethionine to free carboxyl groups of a protein molecule forming methyl esters. EC 2.1.1.-.	Protein Carboxylmethyltransferase,Protein Methylase II,Protein Methyltransferase II,Protein Carboxyl-Methylase,Protein Carboxymethylase,Protein O-Carboxymethyltransferase,Protein-Glutamic(Aspartic)-Methyltransferase,S-Adenosylmethionine Protein Carboxymethyltransferase,S-Adenosylmethionine Protein O-Methyltransferase,S-Adenosylmethionine-Protein Carboxymethyl Transferase,Carboxyl-Methylase, Protein,Carboxylmethyltransferase, Protein,Carboxymethyl Transferase, S-Adenosylmethionine-Protein,Carboxymethylase, Protein,Carboxymethyltransferase, S-Adenosylmethionine Protein,Methylase II, Protein,Methyltransferase II, Protein,O-Carboxymethyltransferase, Protein,O-Methyltransferase, Protein,O-Methyltransferase, S-Adenosylmethionine Protein,Protein Carboxyl Methylase,Protein Carboxymethyltransferase, S-Adenosylmethionine,Protein O Carboxymethyltransferase,Protein O Methyltransferase,Protein O-Methyltransferase, S-Adenosylmethionine,S Adenosylmethionine Protein Carboxymethyl Transferase,S Adenosylmethionine Protein Carboxymethyltransferase,S Adenosylmethionine Protein O Methyltransferase,Transferase, S-Adenosylmethionine-Protein Carboxymethyl
D011950	Receptors, Cholinergic	Cell surface proteins that bind acetylcholine with high affinity and trigger intracellular changes influencing the behavior of cells. Cholinergic receptors are divided into two major classes, muscarinic and nicotinic, based originally on their affinity for nicotine and muscarine. Each group is further subdivided based on pharmacology, location, mode of action, and/or molecular biology.	ACh Receptor,Acetylcholine Receptor,Acetylcholine Receptors,Cholinergic Receptor,Cholinergic Receptors,Cholinoceptive Sites,Cholinoceptor,Cholinoceptors,Receptors, Acetylcholine,ACh Receptors,Receptors, ACh,Receptor, ACh,Receptor, Acetylcholine,Receptor, Cholinergic,Sites, Cholinoceptive
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D004557	Electric Organ	In about 250 species of electric fishes, modified muscle fibers forming disklike multinucleate plates arranged in stacks like batteries in series and embedded in a gelatinous matrix. A large torpedo ray may have half a million plates. Muscles in different parts of the body may be modified, i.e., the trunk and tail in the electric eel, the hyobranchial apparatus in the electric ray, and extrinsic eye muscles in the stargazers. Powerful electric organs emit pulses in brief bursts several times a second. They serve to stun prey and ward off predators. A large torpedo ray can produce of shock of more than 200 volts, capable of stunning a human. (Storer et al., General Zoology, 6th ed, p672)	Electric Organs,Organ, Electric,Organs, Electric
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012436	S-Adenosylmethionine	Physiologic methyl radical donor involved in enzymatic transmethylation reactions and present in all living organisms. It possesses anti-inflammatory activity and has been used in treatment of chronic liver disease. (From Merck, 11th ed)	AdoMet,Ademetionine,FO-1561,Gumbaral,S Amet,S-Adenosyl-L-Methionine,S-Adenosylmethionine Sulfate Tosylate,SAM-e,Samyr,FO 1561,FO1561,S Adenosyl L Methionine,S Adenosylmethionine,S Adenosylmethionine Sulfate Tosylate