Biomaterial Database

Improved mass determination of isolated biological objects by transmission electron microscopy and scanning microdensitometry. 1982

P W Linders, and D J Zahniser, and A L Stols, and A M Stadhouders

A modification of the classical method of Bahr and Zeitler for dry mass determination of isolated biological objects is described. The method uses a conventional transmission electron microscope (TEM) and a computer-controlled scanning microdensitometer. The performance of the modified method has been investigated by applying it to model specimens (polystyrene latex spheres). The method offers significant improvements over the existing TEM-based mass determination method.

UI	MeSH Term	Description	Entries
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D003201	Computers	Programmable electronic devices designed to accept data, perform prescribed mathematical and logical operations at high speed, and display the results of these operations.	Calculators, Programmable,Computer Hardware,Computers, Digital,Hardware, Computer,Calculator, Programmable,Computer,Computer, Digital,Digital Computer,Digital Computers,Programmable Calculator,Programmable Calculators
D003720	Densitometry	The measurement of the density of a material by measuring the amount of light or radiation passing through (or absorbed by) the material.	Densitometries
D014894	Weights and Measures	Measuring and weighing systems and processes.	Measures,Scales,Weights,Measure,Measures and Weights