Biomaterial Database

Lineage analysis of inner cell mass and trophectoderm using microsurgically reconstituted mouse blastocysts. 1982

V E Papaioannou

The fate of mouse blastocyst tissues was examined following reconstitution of blastocysts from isolated inner cell mass (ICM) and trophectoderm differing for electrophoretic variants at the glucose phosphate isomerase (GPI-1) locus. A modified microsurgical method was used and a more sensitive enzyme assay allowed finer dissection of developing chimaeric conceptuses. In seven of nine cases, the extraembryonic ectoderm or the later ectoderm of the chorion was entirely of the blastocyst trophectoderm enzyme type, providing the first direct evidence that this tissue can be wholly derived from the trophectoderm. The two exceptions could represent contamination of the ICM with trophectoderm or might indicate some developmental lability of ICM cells. In addition, the results confirm the cell lineages of other tissues of the 7.5- to 9.5-day pc embryo and, for the first time, directly demonstrate the ICM origin of the parietal endoderm.

UI	MeSH Term	Description	Entries
D001755	Blastocyst	A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.	Embryo, Preimplantation,Blastocysts,Embryos, Preimplantation,Preimplantation Embryo,Preimplantation Embryos
D002449	Cell Aggregation	The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type.	Aggregation, Cell,Aggregations, Cell,Cell Aggregations
D002678	Chimera	An individual that contains cell populations derived from different zygotes.	Hybrids,Chimeras,Hybrid
D004475	Ectoderm	The outer of the three germ layers of an embryo.	Apical Ectodermal Ridge,Apical Ectodermal Ridges,Ectodermal Ridge, Apical,Ectoderms
D004592	Electrophoresis, Starch Gel	Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium.	Starch Gel Electrophoresis
D004707	Endoderm	The inner of the three germ layers of an embryo.	Definitive Endoderm,Definitive Endoderms,Endoderm, Definitive,Endoderms
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014327	Trophoblasts	Cells lining the outside of the BLASTOCYST. After binding to the ENDOMETRIUM, trophoblasts develop into two distinct layers, an inner layer of mononuclear cytotrophoblasts and an outer layer of continuous multinuclear cytoplasm, the syncytiotrophoblasts, which form the early fetal-maternal interface (PLACENTA).	Cytotrophoblasts,Syncytiotrophoblasts,Trophoblast,Cytotrophoblast,Syncytiotrophoblast
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus