Biomaterial Database

The role of intracellular chloride in hyperpolarizing post-synaptic inhibition of crayfish stretch receptor neurones. 1982

R A Deisz, and H D Lux

1. The intracellular Cl(-) activity (a(Cl) (i)) of isolated crayfish stretch receptor neurones was measured using liquid ion exchanger Cl(-)-selective micro-electrodes. The potential developed due to the difference between the normal extracellular Cl(-) activity (a(Cl) (o)) and a(Cl) (i) (V(Cl)) was compared with the simultaneously measured reversal potential of the inhibitory post-synaptic potential (E(i.p.s.p.)) to further clarify the ionic basis of the i.p.s.p..2. In normal Ringer solution, V(Cl) (63.3 +/- 2.3 mV) was found to be close to the resting membrane potential (E(m), 62.6 +/- 3.9 mV) while E(i.p.s.p.) (74.5 +/- 1.9 mV) was more negative than either. The V(Cl) value corresponds to an apparent a(Cl) (i) of 12.7 +/- 1.3 mM, which is about 4 mM more than required for a Cl(-) governed E(i.p.s.p.) of 74.5 mV.3. Reducing a(Cl) (o) caused smaller changes in V(Cl) than predicted for passive Cl(-) re-distributions. On complete removal of extracellular Cl(-) (Cl(o) (-)), V(Cl) increased to 84.6 +/- 2.7 mV, equivalent to an apparent a(Cl) (i) of about 5 mM-Cl(-). This value can be used as an estimate of the level of intracellular interference on the Cl(-)-selective micro-electrode.4. Increasing extracellular K(+) (K(0) (+)) decreased both V(Cl) and E(i.p.s.p.). Decreasing K(o) (+) had the converse effect. The time course of the changes in V(Cl) and E(i.p.s.p.) was much the same. The difference between V(Cl) and E(i.p.s.p.) decreased to about 3 mV in high K(o) (+), and increased to about 30 mV in low K(o) (+). This variation in the difference between E(i.p.s.p.) and V(Cl) is consistent with the assumption that anions other than Cl(-) contribute to the recorded V(Cl) rather than another ion contributes to the inhibitory current.5. Application of 5 mM-NH(4) (+) or of frusemide (6 x 10(-4) M) decreased V(Cl) and E(i.p.s.p.). The difference between V(Cl) and E(i.p.s.p.) was also decreased.6. We conclude that a(Cl) (i) is lower than predicted from a passive distribution and thus the chloride equilibrium potential (E(Cl)) is more negative than E(m). If a constant intracellular interference equivalent to about 4 mM-Cl(-) is assumed to contribute to the recorded V(Cl), E(Cl) was approximately equal to E(i.p.s.p.) in all the experimental conditions. Therefore we suggest that the i.p.s.p. is solely generated by Cl(-) ions.

UI	MeSH Term	Description	Entries
D007424	Intracellular Fluid	The fluid inside CELLS.	Fluid, Intracellular,Fluids, Intracellular,Intracellular Fluids
D008465	Mechanoreceptors	Cells specialized to transduce mechanical stimuli and relay that information centrally in the nervous system. Mechanoreceptor cells include the INNER EAR hair cells, which mediate hearing and balance, and the various somatosensory receptors, often with non-neural accessory structures.	Golgi Tendon Organ,Golgi Tendon Organs,Krause's End Bulb,Krause's End Bulbs,Mechanoreceptor,Mechanoreceptor Cell,Meissner's Corpuscle,Neurotendinous Spindle,Neurotendinous Spindles,Receptors, Stretch,Ruffini's Corpuscle,Ruffini's Corpuscles,Stretch Receptor,Stretch Receptors,Mechanoreceptor Cells,Bulb, Krause's End,Bulbs, Krause's End,Cell, Mechanoreceptor,Cells, Mechanoreceptor,Corpuscle, Meissner's,Corpuscle, Ruffini's,Corpuscles, Ruffini's,End Bulb, Krause's,End Bulbs, Krause's,Krause End Bulb,Krause End Bulbs,Krauses End Bulb,Krauses End Bulbs,Meissner Corpuscle,Meissners Corpuscle,Organ, Golgi Tendon,Organs, Golgi Tendon,Receptor, Stretch,Ruffini Corpuscle,Ruffini Corpuscles,Ruffinis Corpuscle,Ruffinis Corpuscles,Spindle, Neurotendinous,Spindles, Neurotendinous,Tendon Organ, Golgi,Tendon Organs, Golgi
D008564	Membrane Potentials	The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).	Resting Potentials,Transmembrane Potentials,Delta Psi,Resting Membrane Potential,Transmembrane Electrical Potential Difference,Transmembrane Potential Difference,Difference, Transmembrane Potential,Differences, Transmembrane Potential,Membrane Potential,Membrane Potential, Resting,Membrane Potentials, Resting,Potential Difference, Transmembrane,Potential Differences, Transmembrane,Potential, Membrane,Potential, Resting,Potential, Transmembrane,Potentials, Membrane,Potentials, Resting,Potentials, Transmembrane,Resting Membrane Potentials,Resting Potential,Transmembrane Potential,Transmembrane Potential Differences
D009433	Neural Inhibition	The function of opposing or restraining the excitation of neurons or their target excitable cells.	Inhibition, Neural
D011188	Potassium	An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
D002712	Chlorides	Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.	Chloride,Chloride Ion Level,Ion Level, Chloride,Level, Chloride Ion
D003400	Astacoidea	A superfamily of various freshwater CRUSTACEA, in the infraorder Astacidea, comprising the crayfish. Common genera include Astacus and Procambarus. Crayfish resemble lobsters, but are usually much smaller.	Astacus,Crayfish,Procambarus,Astacoideas,Crayfishs
D005665	Furosemide	A benzoic-sulfonamide-furan. It is a diuretic with fast onset and short duration that is used for EDEMA and chronic RENAL INSUFFICIENCY.	Frusemide,Fursemide,Errolon,Frusemid,Furanthril,Furantral,Furosemide Monohydrochloride,Furosemide Monosodium Salt,Fusid,Lasix
D000086	Acetazolamide	One of the CARBONIC ANHYDRASE INHIBITORS that is sometimes effective against absence seizures. It is sometimes useful also as an adjunct in the treatment of tonic-clonic, myoclonic, and atonic seizures, particularly in women whose seizures occur or are exacerbated at specific times in the menstrual cycle. However, its usefulness is transient often because of rapid development of tolerance. Its antiepileptic effect may be due to its inhibitory effect on brain carbonic anhydrase, which leads to an increased transneuronal chloride gradient, increased chloride current, and increased inhibition. (From Smith and Reynard, Textbook of Pharmacology, 1991, p337)	Acetadiazol,Acetazolam,Acetazolamide Sodium, (Sterile),Acetazolamide, Monosodium Salt,Ak-Zol,Apo-Acetazolamide,Diacarb,Diamox,Diuramide,Défiltran,Edemox,Glauconox,Glaupax,Huma-Zolamide,Ak Zol,AkZol,Apo Acetazolamide,ApoAcetazolamide,Huma Zolamide,HumaZolamide
D000644	Quaternary Ammonium Compounds	Derivatives of ammonium compounds, NH4+ Y-, in which all four of the hydrogens bonded to nitrogen have been replaced with hydrocarbyl groups. These are distinguished from IMINES which are RN	Quaternary Ammonium Compound,Ammonium Compound, Quaternary,Ammonium Compounds, Quaternary,Compound, Quaternary Ammonium