Biomaterial Database

Incorporation of polyunsaturated fatty acids into lipids of rainbow trout hepatocytes. 1982

P A Sellner, and J R Hazel

Hepatocytes from 5- or 20 degrees C-acclimated rainbow trout (Salmo gairdneri) were incubated with [1-14C]oleic, -linoleic, or -linolenic acid. Both acclimation groups demonstrated greater incorporation of derivatives from linolenic and linoleic acids into phospholipids when assayed at 5 and 20 degrees C; few derivatives of oleic acid were formed. Cells from cold-acclimated trout, when assayed at 5 degrees C with linolenic acid, incorporated a large proportion of radioactivity into free fatty acids. Analysis of each lipid fraction revealed a relatively specific incorporation of certain fatty acids. For example, "dead end" elongation products of the three substrates were preferentially incorporated into neutral lipids, while delta 6 desaturation products of the three acids were retained in the free fatty acid fraction. Twenty-carbon acid derivatives of linoleic and linolenic acids were directed into the phospholipid fraction. Incorporation of the delta 5 desaturation products was temperature sensitive in cells from cold-acclimated but not warm-acclimated trout. The results suggest that selectivity of incorporation of specific fatty acids into phospholipids may be of importance in restructuring membranes of poikilotherms during thermal adaptation.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D003080	Cold Temperature	An absence of warmth or heat or a temperature notably below an accustomed norm.	Cold,Cold Temperatures,Temperature, Cold,Temperatures, Cold
D005231	Fatty Acids, Unsaturated	FATTY ACIDS in which the carbon chain contains one or more double or triple carbon-carbon bonds.	Fatty Acids, Polyunsaturated,Polyunsaturated Fatty Acid,Unsaturated Fatty Acid,Polyunsaturated Fatty Acids,Acid, Polyunsaturated Fatty,Acid, Unsaturated Fatty,Acids, Polyunsaturated Fatty,Acids, Unsaturated Fatty,Fatty Acid, Polyunsaturated,Fatty Acid, Unsaturated,Unsaturated Fatty Acids
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012487	Salmonidae	A family of anadromous fish comprising SALMON; TROUT; whitefish; and graylings. They are the most important food and game fishes. Their habitat is the northern Atlantic and Pacific, both marine and inland, and the Great Lakes. (Nelson: Fishes of the World, 1976, p97)	Graylings,Thymallus,Whitefish,Salmonids,Grayling,Salmonid
D014018	Tissue Distribution	Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.	Distribution, Tissue,Distributions, Tissue,Tissue Distributions
D014337	Trout	Various fish of the family SALMONIDAE, usually smaller than salmon. They are mostly restricted to cool clear freshwater. Some are anadromous. They are highly regarded for their handsome colors, rich well-flavored flesh, and gameness as an angling fish. The genera Salvelinus, Salmo, and ONCORHYNCHUS have been introduced virtually throughout the world.	Chars,Salvelinus,Char
D050356	Lipid Metabolism	Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.	Metabolism, Lipid