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Titration of metal-freed bovine alpha-lactalbumin with Mg2+ ions causes a two-stepped decrease in the fluorescence quantum yield and a pronounced spectral shift towards shorter wavelengths. It seems to reflect conformational changes induced by the binding of two Mg2+ ions to the protein molecule which results in a transfer of some tryptophan residues from the protein surface into an interior part of the protein in rigid unpolar environment. The Mg2+ association constants evaluated from the fluorimetric Mg2+-titration are 2x10(3) and 2x10(2) M-1. Mg2+ ions in millimolar concentrations almost do not influence the binding of Ca2+ ions to protein. It is assumed that Ca2+ and Mg2+ ions are bound to different sites on alpha-lactalbumin. Mono-calcium, mono-magnesium, bi-magnesium and apo-forms of alpha-lactalbumin are distinct in their fluorescence properties which suggests the difference in the conformation of these forms. The binding of Ca2+ and Mg2+ ions to alpha-lactalbumin has to modulate its function.
E A Permiakov, and
L P Kalinichenko, and
L A Morozova, and
V V Iarmolenko, and
E A Burshteĭn
December 1991,
Journal of protein chemistry,
E A Permiakov, and
L P Kalinichenko, and
L A Morozova, and
V V Iarmolenko, and
E A Burshteĭn
November 2022,
ACS omega,
E A Permiakov, and
L P Kalinichenko, and
L A Morozova, and
V V Iarmolenko, and
E A Burshteĭn
April 1988,
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E A Permiakov, and
L P Kalinichenko, and
L A Morozova, and
V V Iarmolenko, and
E A Burshteĭn
November 2001,
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E A Permiakov, and
L P Kalinichenko, and
L A Morozova, and
V V Iarmolenko, and
E A Burshteĭn
January 1997,
Membrane & cell biology,
E A Permiakov, and
L P Kalinichenko, and
L A Morozova, and
V V Iarmolenko, and
E A Burshteĭn
December 1991,
European journal of biochemistry,
E A Permiakov, and
L P Kalinichenko, and
L A Morozova, and
V V Iarmolenko, and
E A Burshteĭn
June 2004,
Langmuir : the ACS journal of surfaces and colloids,
E A Permiakov, and
L P Kalinichenko, and
L A Morozova, and
V V Iarmolenko, and
E A Burshteĭn
January 2002,
Journal of inorganic biochemistry,
E A Permiakov, and
L P Kalinichenko, and
L A Morozova, and
V V Iarmolenko, and
E A Burshteĭn
July 1989,
Biochimica et biophysica acta,
E A Permiakov, and
L P Kalinichenko, and
L A Morozova, and
V V Iarmolenko, and
E A Burshteĭn
February 1983,
The Biochemical journal,
