Biomaterial Database

Non-heme iron proteins of Desulfovibrio: the primary structure of ferredoxin I from Desulfovibrio africanus. 1982

M Bruschi, and E C Hatchikian

Three different ferredoxins have been isolated from the sulfate reducing bacterium, Desulfovibrio africanus. The present paper describes the complete amino acid sequence of D. africanus ferredoxin I. This sequence was determined using automatic protein sequencing in liquid phase and in solid phase. The 61 amino acid residues of the sequence have been aligned with the aid of peptides obtained by cyanogen bromide, cleavage and by tryptic hydrolysis. This ferredoxin which contains 4 cysteine residues represents the most simple case of one (4 Fe-4 S) cluster ferredoxin. A comparison of D. africanus ferredoxin I with D. gigas and Clostridium pasteurianum ferredoxins is presented in terms of structural and possible evolutionary relationships.

UI	MeSH Term	Description	Entries
D003488	Cyanogen Bromide	Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes.	Bromide, Cyanogen
D003901	Desulfovibrio	A genus of gram-negative, anaerobic, rod-shaped bacteria capable of reducing sulfur compounds to hydrogen sulfide. Organisms are isolated from anaerobic mud of fresh and salt water, animal intestines, manure, and feces.
D005288	Ferredoxins	Iron-containing proteins that transfer electrons, usually at a low potential, to flavoproteins; the iron is not present as in heme. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)	Ferredoxin,Ferredoxin I,Ferredoxin II,Ferredoxin III
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D014357	Trypsin	A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.	Tripcellim,Trypure,beta-Trypsin,beta Trypsin