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Recent interest in the putative protective role of high-density lipoprotein (HDL) and its subfractions against atherosclerosis has highlighted the need for a rapid, simple subfractionation procedure. Here we compared HDL subfractionation by two recently developed polyanionic-precipitation methods with the values obtained by rate zonal ultracentrifugation. A similar result for total HDL cholesterol was obtained by all three methods. However, HDL2 cholesterol as measured by the precipitation procedures was significantly higher than the zonal value, and HDL3 was lower. This reflects the different underlying principles involved in the separations and highlights the need for a clearer understanding of the functional roles of the HDL fractions.
H S Simpson, and
F C Ballantyne, and
C J Packard, and
H G Morgan, and
J Shepherd
February 1975,
FEBS letters,
H S Simpson, and
F C Ballantyne, and
C J Packard, and
H G Morgan, and
J Shepherd
September 1994,
Clinical chemistry,
H S Simpson, and
F C Ballantyne, and
C J Packard, and
H G Morgan, and
J Shepherd
July 1978,
The Journal of biological chemistry,
H S Simpson, and
F C Ballantyne, and
C J Packard, and
H G Morgan, and
J Shepherd
July 1974,
Journal of lipid research,
H S Simpson, and
F C Ballantyne, and
C J Packard, and
H G Morgan, and
J Shepherd
January 1978,
Preparative biochemistry,
H S Simpson, and
F C Ballantyne, and
C J Packard, and
H G Morgan, and
J Shepherd
June 1993,
The American journal of medicine,
H S Simpson, and
F C Ballantyne, and
C J Packard, and
H G Morgan, and
J Shepherd
April 1980,
The Journal of biological chemistry,
H S Simpson, and
F C Ballantyne, and
C J Packard, and
H G Morgan, and
J Shepherd
June 1974,
European journal of biochemistry,
H S Simpson, and
F C Ballantyne, and
C J Packard, and
H G Morgan, and
J Shepherd
January 2006,
Analytical chemistry,
H S Simpson, and
F C Ballantyne, and
C J Packard, and
H G Morgan, and
J Shepherd
July 1990,
Atherosclerosis,
