BIOMAT Database Logo BIOMAT Database Logo

Determination of glycosylated hemoglobin by affinity chromatography: comparison with colorimetric and ion-exchange methods, and effects of common interferences. 1982

D C Klenk, and G T Hermanson, and R I Krohn, and E K Fujimoto, and A K Mallia, and P K Smith, and J D England, and H M Wiedmeyer, and R R Little, and D E Goldstein

An affinity-chromatographic method for determination of glycosylated hemoglobin (Anal. Lett. 14: 649-661, 1981) is compared with the thiobarbituric acid colorimetric (I) (Clin. Chem. 27: 669-672, 1981) and the ion-exchange liquid-chromatographic (II) (Diabetes 29: 623-628, 1980) methods. A correlation of 0.98 was obtained for the affinity method vs II and 0.97 for affinity vs I (n = 51). The within-run CV was 1.9% for specimens from non-diabetic individuals and 1.0% for those from diabetics. The respective between-run CVs were 3.4% and 2.4%. Failure to remove "labile" glucose adducts by 5-h incubation of erythrocytes in isotonic saline (37 degrees C) contributed an average error of 13.1% for II, 5.4% for I, and 1.6% for the affinity method. Affinity chromatography gave a decrease of 0.1-0.2% glycosylated hemoglobin for each 1.0 degree C temperature increase between 18 and 27 degrees C. Varying the pH of the wash buffer used in the affinity procedure from 7.75 to 8.25 (pH 8.0 optimum) produced at net change of 0.5% in glycosylated hemoglobin with one diabetic specimen. Using the affinity method, we determined the reference interval for glycosylated hemoglobin in 124 apparently healthy individuals to be 5.3 to 7.5% (mean 6.36%, SD 0.55%). Rechromatography by II and isoelectric focusing analysis of the fractions obtained by the affinity separation revealed a substantial population of glycosylated hemoglobins not measured by II. The affinity method offers a rapid, simple, precise, and accurate alternative to methods currently in use and gives substantial freedom from many common interferences.

UI MeSH Term Description Entries
D007525 Isoelectric Focusing Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point. Electrofocusing,Focusing, Isoelectric
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D002846 Chromatography, Affinity A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D002852 Chromatography, Ion Exchange Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins. Chromatography, Ion-Exchange,Ion-Exchange Chromatography,Chromatographies, Ion Exchange,Chromatographies, Ion-Exchange,Ion Exchange Chromatographies,Ion Exchange Chromatography,Ion-Exchange Chromatographies
D005260 Female Females
D006442 Glycated Hemoglobin Products of non-enzymatic reactions between GLUCOSE and HEMOGLOBIN (occurring as a minor fraction of the hemoglobin of ERYTHROCYTES.) It generally refers to glycated HEMOGLOBIN A. Hemoglobin A1c (Hb A1c) is hemoglobin A with GLYCATION on a terminal VALINE of the beta chain. Glycated hemoglobin A is used as an index of the average blood sugar level over a lifetime of erythrocytes. Fructated Hemoglobins,Glycohemoglobin,Glycohemoglobin A,Glycohemoglobins,Glycosylated Hemoglobin A,Hb A1c,HbA1,Hemoglobin A(1),Hemoglobin A, Glycosylated,Glycated Hemoglobin A,Glycated Hemoglobin A1c,Glycated Hemoglobins,Glycosylated Hemoglobin A1c,Hb A1,Hb A1a+b,Hb A1a-1,Hb A1a-2,Hb A1b,Hemoglobin, Glycated A1a-2,Hemoglobin, Glycated A1b,Hemoglobin, Glycosylated,Hemoglobin, Glycosylated A1a-1,Hemoglobin, Glycosylated A1b,A1a-1 Hemoglobin, Glycosylated,A1a-2 Hemoglobin, Glycated,A1b Hemoglobin, Glycated,A1b Hemoglobin, Glycosylated,Glycated A1a-2 Hemoglobin,Glycated A1b Hemoglobin,Glycosylated A1a-1 Hemoglobin,Glycosylated A1b Hemoglobin,Glycosylated Hemoglobin,Hemoglobin A, Glycated,Hemoglobin A1c, Glycated,Hemoglobin A1c, Glycosylated,Hemoglobin, Glycated,Hemoglobin, Glycated A1a 2,Hemoglobin, Glycosylated A1a 1,Hemoglobins, Fructated,Hemoglobins, Glycated

Related Publications

D C Klenk, and G T Hermanson, and R I Krohn, and E K Fujimoto, and A K Mallia, and P K Smith, and J D England, and H M Wiedmeyer, and R R Little, and D E Goldstein
Comparison of a colorimetric assay for glycosylated hemoglobin with ion-exchange chromatography.
December 1979, Diabetes,
D C Klenk, and G T Hermanson, and R I Krohn, and E K Fujimoto, and A K Mallia, and P K Smith, and J D England, and H M Wiedmeyer, and R R Little, and D E Goldstein
[Determination of glycosylated hemoglobin using affinity chromatography. A comparison of methods].
January 1990, Zeitschrift fur medizinische Laboratoriumsdiagnostik,
D C Klenk, and G T Hermanson, and R I Krohn, and E K Fujimoto, and A K Mallia, and P K Smith, and J D England, and H M Wiedmeyer, and R R Little, and D E Goldstein
Effects of whole blood storage on results for glycosylated hemoglobin as measured by ion-exchange chromatography, affinity chromatography, and colorimetry.
June 1983, Clinical chemistry,
D C Klenk, and G T Hermanson, and R I Krohn, and E K Fujimoto, and A K Mallia, and P K Smith, and J D England, and H M Wiedmeyer, and R R Little, and D E Goldstein
[Determination of glycosylated hemoglobin by affinity chromatography on boron phenylagarose].
January 1993, Voprosy meditsinskoi khimii,
D C Klenk, and G T Hermanson, and R I Krohn, and E K Fujimoto, and A K Mallia, and P K Smith, and J D England, and H M Wiedmeyer, and R R Little, and D E Goldstein
Comparison between colorimetric and column chromatographic methods for the determination of glycosylated hemoglobin.
May 1984, Journal of the Medical Association of Thailand = Chotmaihet thangphaet,
D C Klenk, and G T Hermanson, and R I Krohn, and E K Fujimoto, and A K Mallia, and P K Smith, and J D England, and H M Wiedmeyer, and R R Little, and D E Goldstein
Measurement of glycosylated hemoglobin by affinity chromatography.
November 1983, Mayo Clinic proceedings,
D C Klenk, and G T Hermanson, and R I Krohn, and E K Fujimoto, and A K Mallia, and P K Smith, and J D England, and H M Wiedmeyer, and R R Little, and D E Goldstein
Comparison of column chromatographic, colorimetric and electrophoretic methods for determination of glycosylated hemoglobin (HbA1).
December 1982, Clinical biochemistry,
D C Klenk, and G T Hermanson, and R I Krohn, and E K Fujimoto, and A K Mallia, and P K Smith, and J D England, and H M Wiedmeyer, and R R Little, and D E Goldstein
Correlation of glycosylated hemoglobin measured by affinity vs ion exchange chromatography with mean blood sugar in pediatric IDDM patients.
October 1991, Diabetes research and clinical practice,
D C Klenk, and G T Hermanson, and R I Krohn, and E K Fujimoto, and A K Mallia, and P K Smith, and J D England, and H M Wiedmeyer, and R R Little, and D E Goldstein
Quantitation of glycosylated hemoglobin by boronate affinity chromatography.
January 1984, Diabetes,
D C Klenk, and G T Hermanson, and R I Krohn, and E K Fujimoto, and A K Mallia, and P K Smith, and J D England, and H M Wiedmeyer, and R R Little, and D E Goldstein
[Interpretation of glycosylated hemoglobin levels obtained by affinity chromatography].
January 1983, Annales de biologie clinique,
Copied contents to your clipboard!