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The "high-performance" liquid-chromatographic method we report for measuring palmitate derived from saturated phospholipids in amniotic fluid is sensitive and precise and possesses a wide dynamic range. Palmitate from nonsurfactant sources is largely excluded. The procedure involves oxidation of the chloroform/methanol-extracted lipids with osmium tetroxide, precipitation of the resulting phosphatides with cold acetone, mild alkaline hydrolysis, derivatization of the liberated fatty acids to phenacyl esters, and reversed-phase chromatography. The method is no less convenient or more time-consuming than measurement of the lecithin/sphingomyelin ratio by thin-layer chromatography. The clinical usefulness of the procedure is indicated by results of a correlative study of 26 amniotic fluids evaluated by "lung profile" tests in a second laboratory. A value for dipalmitoyl lecithin of 13 mg/L or greater indicates fetal lung maturity.
S M Sax, and
J J Moore, and
A Oley, and
J S Amenta, and
J A Silverman
October 1974,
Clinical chemistry,
S M Sax, and
J J Moore, and
A Oley, and
J S Amenta, and
J A Silverman
May 1973,
Scandinavian journal of clinical and laboratory investigation,
S M Sax, and
J J Moore, and
A Oley, and
J S Amenta, and
J A Silverman
December 1981,
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S M Sax, and
J J Moore, and
A Oley, and
J S Amenta, and
J A Silverman
November 1976,
Scandinavian journal of clinical and laboratory investigation,
S M Sax, and
J J Moore, and
A Oley, and
J S Amenta, and
J A Silverman
February 1983,
Clinical chemistry,
S M Sax, and
J J Moore, and
A Oley, and
J S Amenta, and
J A Silverman
January 1967,
Environmental science & technology,
S M Sax, and
J J Moore, and
A Oley, and
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J A Silverman
February 1985,
Clinical biochemistry,
S M Sax, and
J J Moore, and
A Oley, and
J S Amenta, and
J A Silverman
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Geburtshilfe und Frauenheilkunde,
S M Sax, and
J J Moore, and
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J A Silverman
January 1973,
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S M Sax, and
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A Oley, and
J S Amenta, and
J A Silverman
February 1975,
Clinical chemistry,
