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1. When rat liver mitochondria are treated with the non-ionic detergent Triton X-100, the solubilizing effects of the latter are already maximal after a few seconds. 2. We have developed a filtration technique that allows a fast separation of the solubilized and non-solubilized fractions. By means of this technique, we have been able to show differences in the solubilizing effect of Triton X-100 according to the physiological state of mitochondria. 3. In addition, the filtrate may be subjected to further fractionation by ultracentrifugation, by which two filtrate subfractions, supernatant and pellet, may be obtained. 4. Filtrates, supernatants and pellets differ from each other, and from intact mitochondria, in phospholipid and polypeptide composition, and possess a characteristic distribution of enzyme activities; they are also different from the ultrastructural point of view. 5. It is concluded that the combined techniques of short detergent treatments and fast filtration are useful in structural and functional studies of mitochondria and in the selective solubilization of mitochondrial components.
M C Barbero, and
E Rial, and
J J Otamendi, and
J I Gurtubay, and
F M Goñi
February 1979,
Biochemical Society transactions,
M C Barbero, and
E Rial, and
J J Otamendi, and
J I Gurtubay, and
F M Goñi
January 1989,
Acta biochimica Polonica,
M C Barbero, and
E Rial, and
J J Otamendi, and
J I Gurtubay, and
F M Goñi
August 1965,
Bollettino della Societa italiana di biologia sperimentale,
M C Barbero, and
E Rial, and
J J Otamendi, and
J I Gurtubay, and
F M Goñi
November 1972,
Biochimica et biophysica acta,
M C Barbero, and
E Rial, and
J J Otamendi, and
J I Gurtubay, and
F M Goñi
January 1962,
Journal of neurochemistry,
M C Barbero, and
E Rial, and
J J Otamendi, and
J I Gurtubay, and
F M Goñi
September 1979,
The Journal of general virology,
M C Barbero, and
E Rial, and
J J Otamendi, and
J I Gurtubay, and
F M Goñi
January 1963,
Rassegna medica sarda,
M C Barbero, and
E Rial, and
J J Otamendi, and
J I Gurtubay, and
F M Goñi
January 1973,
Acta biologica et medica Germanica,
M C Barbero, and
E Rial, and
J J Otamendi, and
J I Gurtubay, and
F M Goñi
January 1968,
Acta biochimica Polonica,
M C Barbero, and
E Rial, and
J J Otamendi, and
J I Gurtubay, and
F M Goñi
January 1984,
The Journal of pharmacology and experimental therapeutics,
