Biomaterial Database

Biochemical studies on acid phosphatase in human placenta and fetal liver during development. 1982

J Kushari, and I Chaudhury, and D Chaudhury, and M Mukherjea

Activity and a few physiochemical properties of acid phosphatase (ACP) have been studied in different subcellular fractions of developing human placenta and fetal liver. The enzyme activity is discernible in all subfractions of each tissue already in the 6th week of gestation. The activity of the hydrolase in 20,000 g pellet, 105,000 g pellet and in 105,000 g supernatant of the placenta and in all fractions of the liver increases significantly with advancement of pregnancy until 26 weeks and declines in term placenta. However, 800 and 10,000 g pellets of the placenta show a gradual decrease in ACP activity with progression of gestation. Experiment with different inhibitors reveals that each tissue contains at least two types of ACP--a soluble one and a sedimentable one. It appears from the Lineweaver-Burk plot of kinetic study that sedimentable form has low Km and high Vmax values. Ag+, Cu++, Hg++, Co++ and Zn++ are inhibitory, while Fe++ and EDTA are stimulatory to the enzyme. The effect of different detergents, particularly with sodium deoxycholate and Triton X-100, shows the increase of the hydrolase activity, indicating 'latent nature' of the enzyme.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D010920	Placenta	A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).	Placentoma, Normal,Placentome,Placentas,Placentomes
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D005260	Female		Females
D005333	Fetus	The unborn young of a viviparous mammal, in the postembryonic period, after the major structures have been outlined. In humans, the unborn young from the end of the eighth week after CONCEPTION until BIRTH, as distinguished from the earlier EMBRYO, MAMMALIAN.	Fetal Structures,Fetal Tissue,Fetuses,Mummified Fetus,Retained Fetus,Fetal Structure,Fetal Tissues,Fetus, Mummified,Fetus, Retained,Structure, Fetal,Structures, Fetal,Tissue, Fetal,Tissues, Fetal
D005865	Gestational Age	The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated from the onset of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization. It is also estimated to begin from fertilization, estrus, coitus, or artificial insemination.	Embryologic Age,Fetal Maturity, Chronologic,Chronologic Fetal Maturity,Fetal Age,Maturity, Chronologic Fetal,Age, Embryologic,Age, Fetal,Age, Gestational,Ages, Embryologic,Ages, Fetal,Ages, Gestational,Embryologic Ages,Fetal Ages,Gestational Ages
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000135	Acid Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.2.	Acid beta-Glycerophosphatase,Acid beta Glycerophosphatase

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