Two complexes of lakes and canals supplying water for two electric power plants, their steam condensors and an adjoining river were investigated by means of culture methods for the presence of Naegleria fowleri in Poland in the period from 1974 to 1980. Sixty-four strains of N. fowleri were isolated, 13 isolates being virulent for mice when instilled intranasally. These strains were found in the steam condensor of the power station A and in waters polluted with warm water of this plant. Pathogenic N. fowleri strains occurred also in an adjoining river connected with the water system of the power plant. The results show the possible role of the steam condensor A as an incubator and regular source of pollution with pathogenic amoebae for its own system of cooling waters and even the adjoining river.