Biomaterial Database

Isolation of proliferating chondrocytes from bovine growth-plate cartilage by rate-zonal centrifugation in a Ficoll density gradient. 1982

R L Ray, and M G Ehrlich, and H J Mankin

A method has been developed for the isolation of growth-plate chondrocytes with different biochemical properties by rate-zonal centrifugation in a Ficoll density gradient. Bovine calf growth plates were first incubated in F-12 medium in the presence of 3H-thymidine to label proliferating chondrocytes. The tissue was then digested with collagenase and the isolated cells were fractionated in a Ficoll density gradient. The chondrocytes, which sedimented into eleven fractions, were analyzed for 3H-thymidine and inorganic pyrophosphatase activity. Chondrocytes that were maximally labeled with 3H-thymidine and contained maximum pyrophosphatase contents were separated in the most dense, bottom fractions of the gradient. These chondrocytes, which were apparently derived from the zone of proliferating chondrocytes, remained viable. This procedure should be applicable to the study of the biochemical properties of chondrocytes from the different zones of normal and diseased growth plates. CONCLUSIONS A procedure has been developed to separate proliferating chondrocytes from hypertrophic chondrocytes and to isolate the proliferating chondrocytes in a viable state. This procedure should make it possible to study in vitro some of te biochemical processes that are involved in normal endochondral ossification, the regulation of these processes, and the alterations that occur in these processes in the presence of growth disorders and disturbances in endochondral ossification.

UI	MeSH Term	Description	Entries
D011884	Radius	The outer shorter of the two bones of the FOREARM, lying parallel to the ULNA and partially revolving around it.	Radial Tuberosity,Radial Tuberosities,Tuberosities, Radial,Tuberosity, Radial
D002356	Cartilage	A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.	Cartilages
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D002501	Centrifugation, Zonal	Centrifugation using a rotating chamber of large capacity in which to separate cell organelles by density-gradient centrifugation. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Centrifugations, Zonal,Zonal Centrifugation,Zonal Centrifugations
D004838	Epiphyses	The head of a long bone that is separated from the shaft by the epiphyseal plate until bone growth stops. At that time, the plate disappears and the head and shaft are united.	Epiphysis
D005362	Ficoll	A sucrose polymer of high molecular weight.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia