Plasma concentrations of cholesterol and testosterone were determined in White Leghorn hens that had just laid soft-shelled (SS) or shell-less (SL) eggs and compared to those that laid hard-shelled (HS) eggs. Hens were bled at two different ages, at 22 to 34 and 66 to 74 weeks of age. Blood samples were collected in the morning hours (0600 to 1200 hr) for both age groups with an additional evening bleed (1500 to 1900 hr) for the 66 to 74-week-old hens. Both plasma constituents were higher in hens that laid SS or SL eggs when compared to those which laid HS eggs, but differences were statistically significant only for the 66 to 74-week-old hens bled in the morning hours. The results of this study indicate that elevated levels of testosterone and its precursor, cholesterol, may be related to the production of eggs with little or no shell calcification.