Biomaterial Database

[Cytochrome pattern of methylotropic acetic acid bacterium MB 58 as dependent on growth conditions]. 1982

A Steudel, and W Babel

In contrast to methylotrophic bacteria investigated up to now the facultative methylotrophic Bacterium MB 58 (Acetobactersp. MB 58) does not possess a cytochrome aa3-complex, but we could find out cytochrome, cytochrome cco, cytochrome a1, and moreover cytochrome d in dependence on the growth conditions. Cytochrome d was found only in stationary phase of heterotrophic growth. Under methylotrophic growth conditions cytochrome d could be demonstrated only by lowering of the aeration rate during the fermentation, by variation the pH-value of the growth medium from 4.0 to 6.5 and with low growth rates (low dilution rates) during continuous fermentation. The addition of cyanide to the oxidized suspension of bacteria during the registration of the cytochrome-redox-difference spectrum allowed the selective representation of cytochrome d under all conditions even if no identification was possible in the spectrum normally. The oxidation of cytochrome d of Acetobacter sp. MB 58 in the presence of cyanide is an indication of its cyanide insensitivity. The low level of b-type cytochromes could be represented by a special technique for registration of spectra. In this connection a unknown absorption peak at 570 nm was registered. The cyanide insensitivity of cytochrome d from Acetobacter sp. MB 58 and the occurrence of several terminal oxidases is appreciated as a hint for a branched respiratory chain.

UI	MeSH Term	Description	Entries
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D003580	Cytochromes	Hemeproteins whose characteristic mode of action involves transfer of reducing equivalents which are associated with a reversible change in oxidation state of the prosthetic group. Formally, this redox change involves a single-electron, reversible equilibrium between the Fe(II) and Fe(III) states of the central iron atom (From Enzyme Nomenclature, 1992, p539). The various cytochrome subclasses are organized by the type of HEME and by the wavelength range of their reduced alpha-absorption bands.	Cytochrome
D005947	Glucose	A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.	Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000091	Acetobacter	A species of gram-negative bacteria of the family ACETOBACTERACEAE found in FLOWERS and FRUIT. Cells are ellipsoidal to rod-shaped and straight or slightly curved.
D000432	Methanol	A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness.	Alcohol, Methyl,Carbinol,Sodium Methoxide,Wood Alcohol,Alcohol, Wood,Methoxide, Sodium,Methyl Alcohol
D013045	Species Specificity	The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.	Species Specificities,Specificities, Species,Specificity, Species
D013386	Succinates	Derivatives of SUCCINIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,4-carboxy terminated aliphatic structure.	Succinic Acids,Acids, Succinic
D019802	Succinic Acid	A water-soluble, colorless crystal with an acid taste that is used as a chemical intermediate, in medicine, the manufacture of lacquers, and to make perfume esters. It is also used in foods as a sequestrant, buffer, and a neutralizing agent. (Hawley's Condensed Chemical Dictionary, 12th ed, p1099; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1851)	Potassium Succinate,Succinate,1,2-Ethanedicarboxylic Acid,1,4-Butanedioic Acid,Ammonium Succinate,Butanedioic Acid,1,2 Ethanedicarboxylic Acid,1,4 Butanedioic Acid,Succinate, Ammonium,Succinate, Potassium