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The effects of pharmacological alteration of adrenergic transmission on the rate of entrance of dopamine-beta-hydroxylase (DBH) into the circulation were assessed in rats by an immunological method in which the kinetics of recovery of serum DBH activity were measured after depletion of the enzyme by treatment with anti-rat DBH antiserum. Neither alpha-receptor blockade with phenoxybenzamine nor ganglionic blockade with clorisondamine altered the rate by which DBH enters the bloodstream although both treatments markedly altered serum catecholamine levels. Prolonged treatment of newborn rats with guanethidine produced a severe peripheral sympathectomy but only a moderate decrease (30%) in serum DBH levels. In the sympathectomized rats, the rate of entrance of DBH into the circulation was significantly reduced whereas the half-life and rate of degradation of the enzyme was unchanged. These results indicate that the major portion of serum DBH does not enter the circulation by means of exocytotic release of the soluble enzyme.
R Grzanna, and
J T Coyle
January 1974,
Advances in biochemical psychopharmacology,
R Grzanna, and
J T Coyle
May 1973,
Circulation research,
R Grzanna, and
J T Coyle
July 1974,
Journal of clinical pharmacology,
R Grzanna, and
J T Coyle
August 1980,
The Journal of clinical endocrinology and metabolism,
R Grzanna, and
J T Coyle
July 1974,
Psychopharmacology bulletin,
R Grzanna, and
J T Coyle
March 1971,
Circulation research,
R Grzanna, and
J T Coyle
March 1976,
Biochemical pharmacology,
R Grzanna, and
J T Coyle
October 1978,
The Journal of clinical endocrinology and metabolism,
R Grzanna, and
J T Coyle
November 1974,
Proceedings of the National Academy of Sciences of the United States of America,
R Grzanna, and
J T Coyle
May 1974,
Life sciences,
