Biomaterial Database

Pyruvate oxidation by the Reiter strain of Treponema phagedenis. 1982

H A George, and R M Smibert

Spectrophotometric assays of pyruvate oxidation catalyzed by extracts of the Reiter strain of Treponema phagedenis indicated that viologen dyes, flavin nucleotides, and a ferric iron chelate, but not pyridine nucleotides, were utilized as electron acceptors. Benzyl viologen-linked activity partially sedimented during ultracentrifugation and appeared similar to clostridial pyruvate:ferredoxin oxidoreductase with respect to the spectral properties of the enzyme chromophore. Electron carrier activity in treponemal extracts was quantitated by a metronidazole-linked assay in which the oxidation of pyruvate by carrier-depleted extracts led to the reduction of electron carrier in the crude extracts which then reduced metronidazole. The rate of metronidazole reduction was proportional to the amount of electron carrier present in the assay. Electron carrier activity in Triton X-100-solubilized, crude extracts partially purified by DEAE-cellulose chromatography and gel filtration was attributed to a protein possessing the spectral and physical properties of a ferredoxin. A similar protein appeared to be present in extracts of Treponema denticola ST10.

UI	MeSH Term	Description	Entries
D007658	Ketone Oxidoreductases	Oxidoreductases that are specific for KETONES.	Oxidoreductases, Ketone
D008795	Metronidazole	A nitroimidazole used to treat AMEBIASIS; VAGINITIS; TRICHOMONAS INFECTIONS; GIARDIASIS; ANAEROBIC BACTERIA; and TREPONEMAL INFECTIONS.	2-Methyl-5-nitroimidazole-1-ethanol,Bayer 5360,Clont,Danizol,Flagyl,Gineflavir,Metric,MetroGel,Metrodzhil,Metrogyl,Metronidazole Hydrochloride,Metronidazole Monohydrochloride,Metronidazole Phosphate,Metronidazole Phosphoester,Satric,Trichazol,Trichopol,Trivazol,Vagilen,2 Methyl 5 nitroimidazole 1 ethanol
D010084	Oxidation-Reduction	A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).	Redox,Oxidation Reduction
D011773	Pyruvates	Derivatives of PYRUVIC ACID, including its salts and esters.
D004579	Electron Transport	The process by which ELECTRONS are transported from a reduced substrate to molecular OXYGEN. (From Bennington, Saunders Dictionary and Encyclopedia of Laboratory Medicine and Technology, 1984, p270)	Respiratory Chain,Chain, Respiratory,Chains, Respiratory,Respiratory Chains,Transport, Electron
D013880	Thioredoxin-Disulfide Reductase	A FLAVOPROTEIN enzyme that catalyzes the oxidation of THIOREDOXINS to thioredoxin disulfide in the presence of NADP+. It was formerly listed as EC 1.6.4.5	Thioredoxin Reductase (NADPH),NADP-Thioredoxin Reductase,NADPH-Thioredoxin Reductase,Thioredoxin Reductase,NADP Thioredoxin Reductase,NADPH Thioredoxin Reductase,Reductase, NADP-Thioredoxin,Reductase, NADPH-Thioredoxin,Reductase, Thioredoxin,Reductase, Thioredoxin-Disulfide,Thioredoxin Disulfide Reductase
D014208	Treponema	A genus of microorganisms of the order SPIROCHAETALES, many of which are pathogenic and parasitic for man and animals.
D050743	Pyruvate Synthase	A ferredoxin-containing enzyme that catalyzes the COENZYME A-dependent oxidative decarboxylation of PYRUVATE to acetyl-COENZYME A and CARBON DIOXIDE.	Pyruvate Ferredoxin Oxidoreductase,Pyruvate Oxidoreductase,Pyruvate Synthetase,Pyruvate-Ferredoxin Oxidoreductase,Ferredoxin Oxidoreductase, Pyruvate,Oxidoreductase, Pyruvate,Oxidoreductase, Pyruvate Ferredoxin,Oxidoreductase, Pyruvate-Ferredoxin,Synthase, Pyruvate,Synthetase, Pyruvate
D019289	Pyruvic Acid	An intermediate compound in the metabolism of carbohydrates, proteins, and fats. In thiamine deficiency, its oxidation is retarded and it accumulates in the tissues, especially in nervous structures. (From Stedman, 26th ed)	Pyruvate,Acid, Pyruvic