Biomaterial Database

DNA sequence analysis of the region encoding the terminal protein and the hypothetical N-gene product of adenovirus type 2. 1982

P Aleström, and G Akusjärvi, and M Pettersson, and U Pettersson

We have determined the complete nucleotide sequence of a 5783-base pair segment of adenovirus type 2 DNA, located between map coordinates 15.8 and 31.7. This region of the adenovirus-2 genome encodes the three segments of the tripartite leader, the i-leader and the two species of virus-associated RNA. The established sequence reveals two translational reading frames encoded by the viral 1-strand which have remarkably large coding capacities. One of these is located between map coordinates 28.9 and 23.5 and encodes a hypothetical polypeptide with an estimated Mr = 74,000. This polypeptide is in an accompanying paper (Smart, J. E., and Stillman, B. W. (1982) J. Biol. Chem. 257, 13499-13506) identified as the precursor for the terminal protein which is covalently attached to the ends of adenovirus DNA. The second large open translational reading frame starts with an ATG triplet at coordinate 22.9 and encodes a hypothetical polypeptide with a predicted Mr = 120,400. This polypeptide is most likely the product of the N-gene, previously mapped between coordinates 18.0 and 22.5 (Galos, R. S., Williams, J., Binger, M. H., and Flint, S. J. (1979) Cell 17, 945-956). The sequence of the r-strand discloses several open translational reading frames, one of which is located within the i-leader.

UI	MeSH Term	Description	Entries
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D004279	DNA, Viral	Deoxyribonucleic acid that makes up the genetic material of viruses.	Viral DNA
D005796	Genes	A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.	Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D005814	Genes, Viral	The functional hereditary units of VIRUSES.	Viral Genes,Gene, Viral,Viral Gene
D006367	HeLa Cells	The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays.	Cell, HeLa,Cells, HeLa,HeLa Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000260	Adenoviruses, Human	Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-G.	APC Viruses,APC Virus,Adenovirus, Human,Human Adenovirus,Human Adenoviruses
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001482	Base Composition	The relative amounts of the PURINES and PYRIMIDINES in a nucleic acid.	Base Ratio,G+C Composition,Guanine + Cytosine Composition,G+C Content,GC Composition,GC Content,Guanine + Cytosine Content,Base Compositions,Base Ratios,Composition, Base,Composition, G+C,Composition, GC,Compositions, Base,Compositions, G+C,Compositions, GC,Content, G+C,Content, GC,Contents, G+C,Contents, GC,G+C Compositions,G+C Contents,GC Compositions,GC Contents,Ratio, Base,Ratios, Base