Specific cleavage at fibers of a bacteriophage-tail-like bacteriocin, pyocin R1, by successive treatment with organomercurial compounds and trypsin. 1982

Y Shimizu, and T Kumazaki, and S I Ishii

When pretreated with the organomercurial compound that reversibly blocks the adhesion of pyocin R1 to sensitive cells, this bacteriocin became susceptible to trypsin. Trypsinization resulted in the exclusive fragmentation of subunit protein no. 2 among 20 different subunit components and the disappearance of distal knobs from the fibers as well as in irreversible inactivation.

UI MeSH Term Description Entries
D010663 Phenylmercury Compounds Organic mercury compounds in which the mercury is attached to a phenyl group. Often used as fungicides and seed treatment agents. Phenyl Mercury Compounds,Phenylmercurials,Compounds, Phenyl Mercury,Compounds, Phenylmercury,Mercury Compounds, Phenyl
D011709 Pyocins Bacteriocins elaborated by mutant strains of Pseudomonas aeruginosa. They are protein or protein-lipopolysaccharide complexes lethal to other strains of the same or related species. Pyocin,Pyocin 1-4,Pyocin 103,Pyocin F 1,Pyocin F 2,Pyocin F 3,Pyocin P 3,Pyocin R,Pyocin R 1,Pyocin R 2,Pyocin R1,Pyocin S 2,Pyocin 1 4
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D001430 Bacteriocins Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria. Bacteriocin,Lantibiotic,Lantibiotics
D014357 Trypsin A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4. Tripcellim,Trypure,beta-Trypsin,beta Trypsin

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