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Mitogenic transformation of lymphocytes from patients with rheumatoid arthritis (RA) has revealed divergent results in different laboratories. Since the sera used for medium supplementation in these previous studies might have influenced the results, we investigated lymphocyte transformation in serum-free medium in 28 RA-patients and 25 controls. It was shown that a majority of patients with RA responded weakly to PHA. Comparing some of these serum-free cultures with cultures that were set in parallel in 10% ABserum, no difference between patients and controls was observed after transformation in supplemented medium. It is concluded that lymphocyte reactivity to PHA (however not to ConA and PWM) is diminished in patients with RA and that this defect--at least partially--is reversible by addition of serum. The use of parallel cultures in serum-free and serum-containing medium is recommended for evaluation of the lymphocyte mitogenic response.
J S Smolen, and
E J Menzel, and
O Scherak
January 1978,
Biochemical Society transactions,
J S Smolen, and
E J Menzel, and
O Scherak
January 1974,
Annales d'immunologie,
J S Smolen, and
E J Menzel, and
O Scherak
February 1974,
Casopis lekaru ceskych,
J S Smolen, and
E J Menzel, and
O Scherak
January 1974,
Arthritis and rheumatism,
J S Smolen, and
E J Menzel, and
O Scherak
November 1977,
Wiener klinische Wochenschrift,
J S Smolen, and
E J Menzel, and
O Scherak
January 1977,
Clinical and experimental immunology,
J S Smolen, and
E J Menzel, and
O Scherak
March 1980,
Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology,
J S Smolen, and
E J Menzel, and
O Scherak
May 1979,
Archives of dermatological research,
J S Smolen, and
E J Menzel, and
O Scherak
January 1981,
Pathologie-biologie,
J S Smolen, and
E J Menzel, and
O Scherak
January 1976,
Folia clinica internacional,
