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The isolated liver of rats treated with dexamethasone, hydrocortisone, and deoxycorticosterone acetate (DOCA) was perfused with Krebs-Ringer Bicarbonate (KRB). The liver of adrenalectomized rats was also perfused with KRB. The glomerulopressin activity of the ultrafiltrate of the liver perfusates was assayed in the tonic tension contraction (TTC) of isolated stomach fundus from rats. As glomerulopressin is known to be a glucuronide, it was inactivated with beta-glucuronidase to confirm that the effect on the stomach fundus was due to the glomerulopressin and not to an autacoid. It was observed that dexamethasone, hydrocortisone and DOCA inhibit glomerulopressin production, but adrenalectomy had no effect, therefore it can be deduced that the adrenals are not necessary for the production of glomerulopressin.
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June 1952,
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