Corticosterone induced changes in serum hormonal profiles and the key enzymes involved in glycolytic and pentose phosphate pathways were studied in caput, corpus and cauda epididymides of mature male rate (200-250 g body weight). Corticosterone (3.5 mg/100 g body weight sc. for 20 days) treatment was found to depress serum testosterone and prolactin while the gonadotrophins were unaltered. Enzymes of both the glycolytic and pentose phosphate pathways were significantly decreased in caput epididymidis. But in corpus and cauda epididymides only the glycolytic enzymes were reduced. Withdrawal of treatment (for 20 days), resulted in restoration of the glycolytic enzymes to normalcy. Tge serum hormonal profiles were also found to be within the normal range. The pentose phosphate pathway in caput epididymidis showed a significant increase in enzyme activities following withdrawal of treatment. From the present investigation it is clear that hypercorticosteronism had a definite influence on epididymal enzymes involved in carbohydrate metabolism.