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A method was developed for the quantitative determination of free morphine and codeine utilizing multiple ion detection mass fragmentography. Samples were spiked with nalorphine (internal standard), extracted and converted to pentafluoropropionyl (PFP) derivatives prior to GC/MS analysis. The PFP derivatives were separated chromatographically on a 5% OV-1 column. The base peak and molecular ion of the El mass spectra were at m/e 414 and 577; 282 and 445; and 440 and 603, for morphine-PFP, codeine-PFP and nalorphine-PFP, respectively. These multiple ions were monitored and the peak area ratio relative to nalorphine-PFP was used as the basis for quantitation. Other drugs studied were found not to interfere with this method.
N B Wu Chen, and
M I Schaffer, and
R L Lin, and
R J Stein
September 1977,
Acta pharmacologica et toxicologica,
N B Wu Chen, and
M I Schaffer, and
R L Lin, and
R J Stein
October 1983,
Journal of chromatography,
N B Wu Chen, and
M I Schaffer, and
R L Lin, and
R J Stein
January 1985,
Journal of analytical toxicology,
N B Wu Chen, and
M I Schaffer, and
R L Lin, and
R J Stein
July 1983,
Journal of chromatography,
N B Wu Chen, and
M I Schaffer, and
R L Lin, and
R J Stein
December 1982,
Nihon eiseigaku zasshi. Japanese journal of hygiene,
N B Wu Chen, and
M I Schaffer, and
R L Lin, and
R J Stein
September 1973,
Analytical biochemistry,
N B Wu Chen, and
M I Schaffer, and
R L Lin, and
R J Stein
April 1982,
Journal of chromatography,
N B Wu Chen, and
M I Schaffer, and
R L Lin, and
R J Stein
February 1990,
Journal of chromatography,
N B Wu Chen, and
M I Schaffer, and
R L Lin, and
R J Stein
November 1978,
Analytical biochemistry,
N B Wu Chen, and
M I Schaffer, and
R L Lin, and
R J Stein
May 1988,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
