BIOMAT Database Logo BIOMAT Database Logo

Single crystals of 5S rRNA from Thermus thermophilus HB8 and preliminary X-ray diffraction data. 1982

K Morikawa, and M Kawakami, and S Takemura

5S rRNA from extremely thermophilic bacteria, Thermus thermophilus HB8, was crystallized. Using a vapour diffusion method, single crystals were grown with sufficient sizes for x-ray diffraction study. The crystals belong to space group P3(1)21 (or enatiomorph P3(2)21) with a = 99 A, b = 99 A and c = 360 A. A unit cell contains probably 36 molecules corresponding to 6 molecules/asymmetric unit. A smaller pseudo-lattice seems to be constructed with the same lengths for a- and b-axis, and 120 A for c-axis. Reflections were observed up to 25 A on x-ray photographs.

UI MeSH Term Description Entries
D009690 Nucleic Acid Conformation The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape. DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D012335 RNA, Ribosomal The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed) Ribosomal RNA,15S RNA,RNA, 15S
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D013824 Thermus Gram-negative aerobic rods found in warm water (40-79 degrees C) such as hot springs, hot water tanks, and thermally polluted rivers.
D014961 X-Ray Diffraction The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Xray Diffraction,Diffraction, X-Ray,Diffraction, Xray,Diffractions, X-Ray,Diffractions, Xray,X Ray Diffraction,X-Ray Diffractions,Xray Diffractions

Related Publications

K Morikawa, and M Kawakami, and S Takemura
Crystallization and preliminary X-ray diffraction studies of nucleoside diphosphate kinase from Thermus thermophilus HB8.
October 2003, Acta crystallographica. Section D, Biological crystallography,
K Morikawa, and M Kawakami, and S Takemura
Crystallization and preliminary X-ray diffraction studies of a NADH oxidase from Thermus thermophilus HB8.
April 1993, Journal of molecular biology,
K Morikawa, and M Kawakami, and S Takemura
Crystallization and preliminary X-ray diffraction analysis of ribosomal protein L11 methyltransferase from Thermus thermophilus HB8.
May 2003, Acta crystallographica. Section D, Biological crystallography,
K Morikawa, and M Kawakami, and S Takemura
Overproduction, crystallization and preliminary X-ray diffraction analysis of probable ATP sulfurylase from Thermus thermophilus HB8.
September 2003, Acta crystallographica. Section D, Biological crystallography,
K Morikawa, and M Kawakami, and S Takemura
Overproduction, crystallization and preliminary X-ray diffraction analysis of a quinone oxidoreductase from Thermus thermophilus HB8.
August 2002, Acta crystallographica. Section D, Biological crystallography,
K Morikawa, and M Kawakami, and S Takemura
Preliminary X-ray crystallographic study of glucose dehydrogenase from Thermus thermophilus HB8.
May 2007, Acta crystallographica. Section F, Structural biology and crystallization communications,
K Morikawa, and M Kawakami, and S Takemura
Purification, crystallization and preliminary X-ray diffraction study on pyrimidine nucleoside phosphorylase TTHA1771 from Thermus thermophilus HB8.
April 2007, Acta crystallographica. Section F, Structural biology and crystallization communications,
K Morikawa, and M Kawakami, and S Takemura
Crystallization and preliminary X-ray studies of isocitrate dehydrogenase from Thermus thermophilus HB8.
October 1995, Journal of biochemistry,
K Morikawa, and M Kawakami, and S Takemura
The nucleotide sequence of 5S rRNA from an extreme thermophile, Thermus thermophilus HB8.
October 1981, Nucleic acids research,
K Morikawa, and M Kawakami, and S Takemura
Crystallization and preliminary X-ray diffraction studies of a DNA excision repair enzyme, UvrB, from Thermus thermophilus HB8.
March 1999, Acta crystallographica. Section D, Biological crystallography,
Copied contents to your clipboard!