Biomaterial Database

Generation of hybridomas secreting murine reaginic antibodies of anti-DNP specificity. 1980

Z Eshhar, and M Ofarim, and T Waks

High frequency of hybridoma lines secreting large amounts of reaginic anti-DNP antibodies were generated by the fusion of splenic lymphocytes enriched by adoptive transfer of immune cells and the NSI plasmacytoma cell line. The murine IgE molecule binds DNP with high affinity and was specifically purified by immunoabsorption. The 190,000 dalton molecule is composed of a lambda-light chain and an epsilon-heavy chain of an apparent m.w. of about 90,000. Absorption studies with anti-class-specific antisera suggest that some of the hybrid clones secrete also a mixed molecule consisting of lambda- and kappa-light chains in addition to the epsilon-heavy chain. The monoclonal reaginic antibody binds to mast cells or rat basophilic leukemia cells and, upon binding of DNP-protein, triggers an anaphylactic reaction.

UI	MeSH Term	Description	Entries
D007073	Immunoglobulin E	An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).	IgE
D007106	Immune Sera	Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.	Antisera,Immune Serums,Sera, Immune,Serums, Immune
D007116	Immunization, Passive	Transfer of immunity from immunized to non-immune host by administration of serum antibodies, or transplantation of lymphocytes (ADOPTIVE TRANSFER).	Convalescent Plasma Therapy,Immunoglobulin Therapy,Immunotherapy, Passive,Normal Serum Globulin Therapy,Passive Antibody Transfer,Passive Transfer of Immunity,Serotherapy,Passive Immunotherapy,Therapy, Immunoglobulin,Antibody Transfer, Passive,Passive Immunization,Therapy, Convalescent Plasma,Transfer, Passive Antibody
D007938	Leukemia	A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)	Leucocythaemia,Leucocythemia,Leucocythaemias,Leucocythemias,Leukemias
D008214	Lymphocytes	White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.	Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008297	Male		Males
D008810	Mice, Inbred C57BL	One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases.	Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D009578	Nitrobenzenes	BENZENE derivatives carrying nitro group substituents.
D011935	Reagins	Antibodies, especially IGE, that bind to tissue of the same species so that ANTIGENS induce HISTAMINE RELEASE and other vasoactive agents. HYPERSENSITIVITY is the clinical manifestation.	Homocytotropic Antibodies,Reagin
D002459	Cell Fusion	Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.	Cell Fusions,Fusion, Cell,Fusions, Cell