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1. The thermotropic behaviour of phospholipid molecules in reconstituted glycophorin-containing vesicles has been investigated by means of differential scanning calorimetry. Each glycophorin molecule is able to perturb the properties of 80--100 phospholipid molecules in such a way that these lipid molecules no longer participate in the cooperative gel to liquid-crystalline phase transition. This number of perturbed phospholipid molecules was discovered to be independent of the lipid charge. 2. By means of freeze-facture electron microscopy it could be demonstrated that glycophorin is not excluded from the solid lipid phase upon cooling the lipids below their gel to liquid-crystalline phase transition temperature. In mixtures of phosphatidylcholines which show solid-solid immiscibility, glycophorin is preferentially associated with the lower-melting lipid component upon phase separation, as could be demonstrated by both differential scanning calorimetry and freeze-fracture electron microscopy. 3. The effect of glycophorin on the mobility of phospholipids has been investigated by means of 31 P NMR. Glycophorin, incorporated into sonicated vesicles of dioleoylphosphatidic acid, is able to immobilize nine lipid molecules very strongly in their phosphate region. Evidence for an electrostatic inter-action between the protein and this negatively charged phospholipid has been presented. 4. The presence of glycophorin causes discontinuities in the lipid bilayer. This results in higher susceptibility of the bilayer towards attack by lipolytic enzymes and in enhanced membrane permeability.
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