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Methyl phosphotriesters have been introduced into DNA, in vitro, by reaction with N-methyl-N-nitrosourea and the rate of degradation in alkali has been followed by measurements of the mean sedimentation coefficient using an analytical ultracentrifuge. In 0.3 M NaOH/0.7 M NaCl, a solution commonly used in alkaline sucrose gradient experiments, hydrolysis of the methyl phosphotriesters present is complete after 15 h at 20 degrees C, or after 2--3 h at 37 degrees C. In addition to breaks formed by the latter reaction there was a continuous background degradation of the DNA giving rise to 6.3 and 63 breaks/10(6) nucleotides per h at 20 degrees and 37 degrees C, respectively. The problem of obtaining quantitative data on phosphotriester concentrations from results of alkaline sucrose gradient experiments has been discussed.
K V Shooter, and
R K Merrifield
May 1982,
Biochimica et biophysica acta,
K V Shooter, and
R K Merrifield
May 1976,
Chemico-biological interactions,
K V Shooter, and
R K Merrifield
December 1973,
Proceedings of the National Academy of Sciences of the United States of America,
K V Shooter, and
R K Merrifield
September 2016,
The Journal of organic chemistry,
K V Shooter, and
R K Merrifield
April 1973,
Journal of molecular biology,
K V Shooter, and
R K Merrifield
December 1977,
Chemico-biological interactions,
K V Shooter, and
R K Merrifield
June 1979,
Biochimica et biophysica acta,
K V Shooter, and
R K Merrifield
October 1993,
American biotechnology laboratory,
K V Shooter, and
R K Merrifield
May 2018,
Chemosphere,
K V Shooter, and
R K Merrifield
February 1982,
Analytical biochemistry,
