Biomaterial Database

Inhibition of chicken erythrocyte AMP deaminase by tetraiodofluorescein compounds. 1978

M Yoshino, and Y Kawamura

A kinetic study has been performed on the inhibition of the chicken erythrocyte AMP deaminase (AMP aminohydrolase, EC 3.5.4.6) reaction by tetraiodofluorescein and Rose Bengal. These dyes inhibited the enzyme by decreasing its affinity for the substrate without affecting the maximum velocity. Kinetic analysis has shown the inhibition constants for tetraiodofluorescein and Rose Bengal to be 350 and 55 micrometer, respectively, and the presence of 4 binding sites of the enzyme for the inhibitors per enzyme molecule. These results suggest that the fluorescein dyes mimic the AMP binding at the catalytic center of the enzyme, which can be formed by the "dinucleotide fold".

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D009709	Nucleotide Deaminases	Catalyze the hydrolysis of nucleotides with the elimination of ammonia.	Deaminases, Nucleotide
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D004923	Erythrosine	A tetraiodofluorescein used as a red coloring in some foods (cherries, fish), as a disclosure of DENTAL PLAQUE, and as a stain of some cell types. It has structural similarity to THYROXINE.	FD & C Red No. 3,2',4',5',7'-Tetraiodofluorescein,Erythrosin,Erythrosin B,Erythrosine B,F D & C #3,FDC Red No. 3
D005452	Fluoresceins	A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.	Tetraiodofluorescein
D000659	AMP Deaminase	An enzyme that catalyzes the deamination of AMP to IMP. EC 3.5.4.6.	AMP Aminase,Adenylate Deaminase,5'-AMP Deaminase,AMP Aminohydrolase,Myoadenylate Deaminase,5' AMP Deaminase,Aminase, AMP,Aminohydrolase, AMP,Deaminase, 5'-AMP,Deaminase, AMP,Deaminase, Adenylate,Deaminase, Myoadenylate
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001665	Binding Sites	The parts of a macromolecule that directly participate in its specific combination with another molecule.	Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D012395	Rose Bengal	A bright bluish pink compound that has been used as a dye, biological stain, and diagnostic aid.	Rose Bengal Sodium I 125,Rose Bengal Sodium I 131,Bengal, Rose