Biomaterial Database

Binding of cytochalasin B to human erythrocyte glucose transporter. 1980

D C Sogin, and P C Hinkle

Cytochalasin B, a potent inhibitor of D-glucose transport systems, binds to the glucose transporter purified from human erythrocytes as described previously [Kasahara, M., & Hinkle, P. C. (1977) J. Biol. Chem. 252, 7384]. The transporter binds 9.2 +/- 1.3 nmol of cytochalasin B/mg of protein with a dissociation constant of 0.18 microM. The binding is competitively inhibited by D-glucose (Ki = 43 mM). Phloretin, diethylstilbestrol, maltose, 6-O-propyl-D-galactose, propyl beta-D-glucopyranoside, and dithiothreitol were also linear competitive inhibitors of cytochalasin B binding. The propyl sugars have been shown to inhibit transport from either the plasma or cytoplasma side of the membrane, respectively. The binding of cytochalasin B to the isolated transporter was inhibited by both propyl sugars.

UI	MeSH Term	Description	Entries
D009004	Monosaccharide Transport Proteins	A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.	Hexose Transport Proteins,Band 4.5 Preactin,Erythrocyte Band 4.5 Protein,Glucose Transport-Inducing Protein,Hexose Transporter,4.5 Preactin, Band,Glucose Transport Inducing Protein,Preactin, Band 4.5,Proteins, Monosaccharide Transport,Transport Proteins, Hexose,Transport Proteins, Monosaccharide,Transport-Inducing Protein, Glucose
D009005	Monosaccharides	Single chain carbohydrates that are the most basic units of CARBOHYDRATES. They are typically colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn.	Monosaccharide,Simple Sugar,Simple Sugars,Sugar, Simple,Sugars, Simple
D011485	Protein Binding	The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.	Plasma Protein Binding Capacity,Binding, Protein
D002352	Carrier Proteins	Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes.	Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D003571	Cytochalasin B	A cytotoxic member of the CYTOCHALASINS.	Phomin
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001667	Binding, Competitive	The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.	Competitive Binding
D001692	Biological Transport	The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.	Transport, Biological,Biologic Transport,Transport, Biologic