The effect of a pattern of exercise and dietary modifications, which was designed to produce alterations in the muscle glycogen content, on the capacity to perform anaerobic exercise was investigated. Six young male subjects worked to exhaustion on a bicycle ergometer at a supramaximal work load equivalent to 104 +/- 5% of VO2max after a normal diet, after a carbohydrate (CHO)-free diet following prolonged exhausting exercise, and after a high-CHO diet. This regimen has previously been shown to cause changes in the glycogen content of the working muscle. Mean work time for subjects on the first test was 4.87 +/- 1.07 min (mean +/- SD). After the low-CHO diet, the time for which work could be maintained was reduced in an increase to 3.32 +/- 0.93 min, whereas administration of the high-CHO diet resulted in an increase to 6.65 +/- 1.39 min. The resting blood lactate concentration was lower than normal following the low-CHO diet and higher than normal following the high-CHO diet. Post exercise blood lactate concentrations reached a peak between 2 and 6 min after exhaustion and again were lower (8.60 +/- 1.58 mmol/l) after the low-CHO diet and higher (12.86 +/- 1.42 mmol/l) after the high-CHO diet than after performing the same intensity of work to exhaustion on a normal diet (11.66 +/- 1.16 mmol/l). The rate of lactate accumulation appeared to be approximately the same during exercise under all three dietary conditions. If this is the case, it suggests that the alterations in endurance capacity do not result from changes in the rate of anaerobic glycolytic energy production, but possibly from a change in the total capacity of the system.