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We present a radioimmunoassay for lysozyme in human serum, based upon human lysozyme isolated from the urine of leukemic patients and antiserum prepared against this lysozyme in the goat. In the separation step, a second antibody is used. By properly adjusting the concentrations of unlabeled and 125I-labeled lysozyme and of the antibodies, maximal precision (SD, 0.04 mg/litre) was obtained in the range 0.00 to 2.00 mg/litre. In 20 normal volunteers the lysozyme concentration was 4.6 +/- 0.8 mg/litre (mean +/- SD), in 13 patients with monocytic leukemia 34.4 +/- 8.6 mg/litre. Correlation with lysoplate determinations was excellent in leukemic sera (r = 0.97) but was poor in normal sera (r = 0.35), possibly owing to the existence of isoenzymes.
T L Peeters, and
Y R Depraetere, and
G R Vantrappen
July 1981,
Clinical chemistry,
T L Peeters, and
Y R Depraetere, and
G R Vantrappen
January 1992,
Journal of pharmaceutical and biomedical analysis,
T L Peeters, and
Y R Depraetere, and
G R Vantrappen
March 1973,
Acta endocrinologica,
T L Peeters, and
Y R Depraetere, and
G R Vantrappen
September 1974,
The Tohoku journal of experimental medicine,
T L Peeters, and
Y R Depraetere, and
G R Vantrappen
January 1971,
Israel journal of medical sciences,
T L Peeters, and
Y R Depraetere, and
G R Vantrappen
July 1993,
Annals of clinical biochemistry,
T L Peeters, and
Y R Depraetere, and
G R Vantrappen
May 1983,
Clinical and experimental immunology,
T L Peeters, and
Y R Depraetere, and
G R Vantrappen
June 1974,
Polskie Archiwum Medycyny Wewnetrznej,
T L Peeters, and
Y R Depraetere, and
G R Vantrappen
May 1990,
Journal of pharmaceutical sciences,
T L Peeters, and
Y R Depraetere, and
G R Vantrappen
August 1978,
Clinica chimica acta; international journal of clinical chemistry,
