Biomaterial Database

Purification and properties of a protease from Lupinus angustifolius during germination. 1978

D V Shepard, and K G Moore

A protease, present in Lupinus angustifolius cotyledons on the fifth day after germination and assayable by following the release of amino groups from gliadin has been purified to the degree that the associated protein of the extract is undetectable. The enzyme is capable, under varying conditions, of releasing amino groups from lupin alpha, beta and gamma conglutins and possesses a mean molecular weight, by dodecylsulphate/polyacrylamide gel electrophoresis and Sephadex G-75 gel filtration of 27500 +/- 450. The isoelectric point is 9.0 +/- 0.848 with a pH optimum of pH 4.0 using gliadin as substrate.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D010447	Peptide Hydrolases	Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.	Peptidase,Peptidases,Peptide Hydrolase,Protease,Proteases,Proteinase,Proteinases,Proteolytic Enzyme,Proteolytic Enzymes,Esteroproteases,Enzyme, Proteolytic,Hydrolase, Peptide
D010944	Plants	Multicellular, eukaryotic life forms of kingdom Plantae. Plants acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations. It is a non-taxonomical term most often referring to LAND PLANTS. In broad sense it includes RHODOPHYTA and GLAUCOPHYTA along with VIRIDIPLANTAE.	Plant
D018521	Plant Physiological Phenomena	The physiological processes, properties, and states characteristic of plants.	Plant Physiological Processes,Plant Physiology,Physiology, Plant,Plant Physiologic Phenomena,Plant Physiologic Phenomenon,Plant Physiological Phenomenon,Plant Physiological Process,Phenomena, Plant Physiologic,Phenomena, Plant Physiological,Phenomenon, Plant Physiologic,Phenomenon, Plant Physiological,Phenomenons, Plant Physiological,Physiologic Phenomena, Plant,Physiologic Phenomenon, Plant,Physiological Phenomena, Plant,Physiological Phenomenon, Plant,Physiological Phenomenons, Plant,Physiological Process, Plant,Physiological Processes, Plant,Plant Physiological Phenomenons,Process, Plant Physiological,Processes, Plant Physiological