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This paper describes a feasibility study for computer image analysis of corneal endothelial specular micrographs. The analysis involved image enhancement, boundary detection of individual cells, and subsequent calculation of cell area, cell area distribution, cell perimeter, cell orientation, and cell diameter. Cell areas produced by automated analysis agreed well with manually measured cell areas from the same photomicrographs. The computer methods required little human guidance or correction, produced accurate results, and could be made to run fairly quickly with modest computing resources.
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