Biomaterial Database

High pressure liquid chromatographic determination of sodium fluoroacetate (compound 1080) in canine gastric content. 1981

A C Ray, and L O Post, and J C Reagor

A sensitive high pressure liquid chromatographic method was developed for the determination of sodium fluoroacetate (compound 1080) in canine gastric content. The procedure involves extraction of 1080 with water, methyl ethyl ketone, and dilute base, followed by sample cleanup using octadecylsilane bonded phase cartridges and derivatization in ethyl acetate sodium with O-p-nitrobenzyl-N-N'-diisopropylisourea (PNBDI). The compound was chromatographed on a 10 micrometer silica column, and ultraviolet absorbance at 254 and 280 nm was measured. Recovery was greater than 95% for standard 1080 and in the 70-90% range for spiked samples (1-50 ppm).

UI	MeSH Term	Description	Entries
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004285	Dogs	The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)	Canis familiaris,Dog
D005463	Fluoroacetates	Derivatives of acetic acid with one or more fluorines attached. They are almost odorless, difficult to detect chemically, and very stable. The acid itself, as well as the derivatives that are broken down in the body to the acid, are highly toxic substances, behaving as convulsant poisons with a delayed action. (From Miall's Dictionary of Chemistry, 5th ed)
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012378	Rodenticides	Substances used to destroy or inhibit the action of rats, mice, or other rodents.	Rodenticide
D013056	Spectrophotometry, Ultraviolet	Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Ultraviolet Spectrophotometry
D013270	Stomach	An organ of digestion situated in the left upper quadrant of the abdomen between the termination of the ESOPHAGUS and the beginning of the DUODENUM.	Stomachs