-transparent.png){kind=logo}
The production of procollagen molecules by human diploid fetal lung fibroblasts (HFL-1 cells) remains constant in both rapid and stationary growth phases. However, log phase cells degrade 3-fold more newly synthesized collagen inside the cell prior to secretion than do stationary phase cells. Procollagen mRNA levels, measured by hybridization with a type I procollagen mRNA-specific complementary DNA, are approximately 2-fold higher in confluent cells than in log phase cells. There are no significant differences in the ability of either log phase or confluent HFL-1 cell procollagen mRNA to be translated in an in vitro cell-free translation system. Therefore, the ability of HFL-1 cells to maintain constant collagen production irrespective of the growth status of the cells results from the combined action of a number of regulatory mechanisms, including changes in procollagen mRNA levels, the utilization of procollagen mRNA, and intracellular procollagen degradation.
P Tolstoshev, and
R A Berg, and
S I Rennard, and
K H Bradley, and
B C Trapnell, and
R G Crystal
May 1990,
Archives of dermatology,
P Tolstoshev, and
R A Berg, and
S I Rennard, and
K H Bradley, and
B C Trapnell, and
R G Crystal
September 1981,
The Journal of biological chemistry,
P Tolstoshev, and
R A Berg, and
S I Rennard, and
K H Bradley, and
B C Trapnell, and
R G Crystal
March 1981,
Cancer research,
P Tolstoshev, and
R A Berg, and
S I Rennard, and
K H Bradley, and
B C Trapnell, and
R G Crystal
September 1998,
American journal of respiratory cell and molecular biology,
P Tolstoshev, and
R A Berg, and
S I Rennard, and
K H Bradley, and
B C Trapnell, and
R G Crystal
August 1977,
Proceedings of the National Academy of Sciences of the United States of America,
P Tolstoshev, and
R A Berg, and
S I Rennard, and
K H Bradley, and
B C Trapnell, and
R G Crystal
April 1977,
Proceedings of the National Academy of Sciences of the United States of America,
P Tolstoshev, and
R A Berg, and
S I Rennard, and
K H Bradley, and
B C Trapnell, and
R G Crystal
November 1972,
Proceedings of the National Academy of Sciences of the United States of America,
P Tolstoshev, and
R A Berg, and
S I Rennard, and
K H Bradley, and
B C Trapnell, and
R G Crystal
April 1987,
The Journal of clinical investigation,
P Tolstoshev, and
R A Berg, and
S I Rennard, and
K H Bradley, and
B C Trapnell, and
R G Crystal
November 1991,
Cornea,
P Tolstoshev, and
R A Berg, and
S I Rennard, and
K H Bradley, and
B C Trapnell, and
R G Crystal
April 1988,
The Journal of investigative dermatology,
