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It had previously been shown that dissociated cell cultures from chick embryo spinal cord have a high affinity uptake system for the neurotransmitter gamma-aminobutyric acid (GABA) and make functional inhibitory synaptic contacts as determined by electrophysiology (Farb et al., 1979). It is shown here that these cultures can synthesize GABA from added glutamate in a glutamate decarboxylase-dependent reaction. Furthermore, these cultures have a functional GABA transaminase that degrades the neurotransmitter. This enzyme can be specifically and irreversibly blocked with gabaculine. A 15 min incubation with 10(-6) M-gabaculine completely inactivates the enzyme. The inactivation of the enzyme leads to an increase in GABA levels. Long-term incubation (16 days) of gabaculine in the medium does not appear to alter high affinity GABA transport, suggesting that the drug is not toxic to cells capable of accumulating GABA.
R R Rando, and
F W Bangerter, and
D H Farb
January 1983,
Developments in biological standardization,
R R Rando, and
F W Bangerter, and
D H Farb
December 1978,
Proceedings of the National Academy of Sciences of the United States of America,
R R Rando, and
F W Bangerter, and
D H Farb
January 1998,
Ryoikibetsu shokogun shirizu,
R R Rando, and
F W Bangerter, and
D H Farb
March 1979,
The Journal of cell biology,
R R Rando, and
F W Bangerter, and
D H Farb
December 1986,
Proceedings of the National Academy of Sciences of the United States of America,
R R Rando, and
F W Bangerter, and
D H Farb
October 1972,
Journal de physiologie,
R R Rando, and
F W Bangerter, and
D H Farb
September 1981,
Biochemical and biophysical research communications,
R R Rando, and
F W Bangerter, and
D H Farb
March 2008,
Brain research,
R R Rando, and
F W Bangerter, and
D H Farb
June 2000,
Experimental neurology,
R R Rando, and
F W Bangerter, and
D H Farb
December 1961,
The Journal of biological chemistry,
