Biomaterial Database

Quantitation of the antineoplastic agent indicine-N-oxide in human plasma by differential pulse polarography. 1980

M McComish, and I Bodek, and A R Branfman

A sensitive and reproducible differential pulse polarographic method of analysis was developed for indicine-N-oxide (NSC 132319) in human plasma. Lyophilized plasma is extracted with methanol, and the extract is chromatographed over partially deactivated aluminum oxide and reversed-phase silica gel columns. Indicine-N-oxide is eluted from the silica gel column with 25% aqueous methanol and quantitated by differential pulse polarography by measurement of the peak current at -0.72 +/- 0.03 v (versus the saturated calomel electrode). Recovery of indicine-N-oxide from plasma was 88 +/- 7% (SD) in the 1-20-microgram/ml range. The method was linear over the range of 0.5-10 microgram/ml of pH 4.6 buffer.

UI	MeSH Term	Description	Entries
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D011048	Polarography	An electrochemical technique for measuring the current that flows in solution as a function of an applied voltage. The observed polarographic wave, resulting from the electrochemical response, depends on the way voltage is applied (linear sweep or differential pulse) and the type of electrode used. Usually a mercury drop electrode is used.	DC Polarography,Pulse Polarography,Polarography, DC,Polarography, Pulse
D011763	Pyrrolizidine Alkaloids	A group of ALKALOIDS, characterized by a nitrogen-containing necine, occurring mainly in plants of the BORAGINACEAE; COMPOSITAE; and LEGUMINOSAE plant families. They can be activated in the liver by hydrolysis of the ester and desaturation of the necine base to reactive electrophilic pyrrolic CYTOTOXINS.	Pyrrolizidine Alkaloid,Senecio Alkaloid,Senecio Alkaloids,Alkaloid, Pyrrolizidine,Alkaloid, Senecio,Alkaloids, Pyrrolizidine,Alkaloids, Senecio
D003497	Cyclic N-Oxides	Heterocyclic compounds in which an oxygen is attached to a cyclic nitrogen.	Heterocyclic N-Oxides,Cyclic N Oxides,Heterocyclic N Oxides,N Oxides, Cyclic,N-Oxides, Cyclic,N-Oxides, Heterocyclic,Oxides, Cyclic N
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000972	Antineoplastic Agents, Phytogenic	Agents obtained from higher plants that have demonstrable cytostatic or antineoplastic activity.	Antineoplastics, Botanical,Antineoplastics, Phytogenic,Agents, Phytogenic Antineoplastic,Botanical Antineoplastics,Phytogenic Antineoplastic Agents,Phytogenic Antineoplastics