Biomaterial Database

Development and application of a radioimmunoassay for tetracycline. 1981

B A Faraj, and F M Ali

Antiserum against tetracycline was produced in rabbits immunized with a tetracycline hapten conjugated to bovine serum albumin. The antiserum was used to develop a radioimmunoassay for tetracycline. As little was 1 ng of tetracycline can be detected in one sample. The antitetracycline antiserum showed a considerable degree of specificity since it cross-reacted only with chlortetracycline (70%). However, its cross-reactivity with oxytetracycline, doxycycline, demeclocycline, methacycline and rolitetracycline was only negligible. The radioimmunoassay of tetracycline was used to measure the systemic bioavailability of orally administered tetracycline (25 mg/kg) to a group of mongrel dogs. Detectable levels of tetracycline were exhibited within 30 min and maximum plasma concentrations of the drug occurred within 3 to 4 hr after dose administration.

UI	MeSH Term	Description	Entries
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D004285	Dogs	The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)	Canis familiaris,Dog
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000918	Antibody Specificity	The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.	Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D013754	Tetracyclines	Closely congeneric derivatives of the polycyclic naphthacenecarboxamide. (Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1117)