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A new set of am and ts-mutants of the phage phi80 was isolated. A series of lambdacI857h80att80int3 prophage deletion strains, extending for various lengths into the region of structural genes was generated and characterized in order to facilitate the mapping of the phi80 am and ts-mutants. Complementation test allowed to find out 5 new genes named 20, 21, 22, 23 and 24. The result of the deletion mapping of structural genes is as follows: 1-2-3-20-4-5-6-21-7-22-9-23-10-8-12-24-13.
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