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The isolated hepatocyte suspension was evaluated as an experimental procedure for investigating liver iron metabolism. Following prelabelling in vivo with transferrin-59Fe, isolated hepatocytes released radioactive iron in vitro by a temperature dependent process, without change in cell viability. Iron mobilization was increased by serum, apotransferrin and a range of iron chelators, of which the most effective were citrate, desferrioxamine and the ionophore A 23187. The rate of iron release was inversely related to oxygen levels, indicating that a ferric-ferrous reduction was involved in iron mobilization. The uncoupler TTFB, DTPA, and hypercapnia caused a reduction in iron release, but the metabolites cysteine, NADH and ascorbic acid had no effect. It was concluded that isolated hepatocytes are a useful experimental model for studying iron metabolism and for further evaluation of iron chelators.
E Baker, and
F R Vicary, and
E R Huehns
January 1986,
The International journal of biochemistry,
E Baker, and
F R Vicary, and
E R Huehns
June 1984,
Biochimica et biophysica acta,
E Baker, and
F R Vicary, and
E R Huehns
January 1985,
The American journal of physiology,
E Baker, and
F R Vicary, and
E R Huehns
January 1981,
Hepatology (Baltimore, Md.),
E Baker, and
F R Vicary, and
E R Huehns
October 1995,
The Biochemical journal,
E Baker, and
F R Vicary, and
E R Huehns
October 1986,
Toxicology,
E Baker, and
F R Vicary, and
E R Huehns
June 1977,
Biochemical and biophysical research communications,
E Baker, and
F R Vicary, and
E R Huehns
December 1984,
Biochemical and biophysical research communications,
E Baker, and
F R Vicary, and
E R Huehns
December 1991,
The Journal of biological chemistry,
E Baker, and
F R Vicary, and
E R Huehns
November 1989,
Journal of applied physiology (Bethesda, Md. : 1985),
