Biomaterial Database

Radioimmunoassay of gentamicin in microliter and nanoliter samples of biological fluids. 1981

A Meulemans, and C Manuel, and P Tran Ba Huy

A gentamicin radioimmunoassay is described for microliter and nanoliter samples. The assay was performed in the inner ears' fluids (perilymph and endolymph) of rats. Endolymph and perilymph samples were collected under oil with a glass capillary micropipette. The absolute threshold of sensitivity was 20 . 10(-12) g. Considering the maximum sampling volume, 15--400 . 10(-9) and 10 . 16(-6) l for endolymph and perilymph respectively, the limit of detection is 4.19 . 10(-4) microM . ml-1 in endolymph, 1.26 . 10(-4) microM . ml-1 in perilymph. Clinical applications of this method are suggested.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008196	Lymph	The interstitial fluid that is in the LYMPHATIC SYSTEM.	Lymphs
D008832	Microchemistry	The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D001826	Body Fluids	Liquid components of living organisms.	Body Fluid,Fluid, Body,Fluids, Body
D005839	Gentamicins	A complex of closely related aminoglycosides obtained from MICROMONOSPORA purpurea and related species. They are broad-spectrum antibiotics, but may cause ear and kidney damage. They act to inhibit PROTEIN BIOSYNTHESIS.	Gentamicin Sulfate (USP),Gentamycin,G-Myticin,Garamycin,Gentacycol,Gentamicin,Gentamicin Sulfate,Gentamycins,Gentavet,Genticin,G Myticin,GMyticin,Sulfate, Gentamicin
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus