Biomaterial Database

A direct spectrophotometric assay for branched-chain 2-oxo acid dehydrogenase activity in intact rat liver mitochondria. 1981

K Bartlett, and H S Sherratt

UI	MeSH Term	Description	Entries
D007658	Ketone Oxidoreductases	Oxidoreductases that are specific for KETONES.	Oxidoreductases, Ketone
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008930	Mitochondria, Liver	Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4)	Liver Mitochondria,Liver Mitochondrion,Mitochondrion, Liver
D009097	Multienzyme Complexes	Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.	Complexes, Multienzyme
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013053	Spectrophotometry	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
D042942	3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)	A ketone oxidoreductase that catalyzes the overall conversion of alpha-keto acids to ACYL-CoA and CO2. The enzyme requires THIAMINE DIPHOSPHATE as a cofactor. Defects in genes that code for subunits of the enzyme are a cause of MAPLE SYRUP URINE DISEASE. The enzyme was formerly classified as EC 1.2.4.3.	2-Oxoisovalerate Dehydrogenase (Lipoamide),2-Oxoisocaproate Dehydrogenase,Alpha-Keto Acid Dehydrogenase,BCKA Decarboxylase,Branched Chain Alpha-Keto Acid Decarboxylase,Branched Chain Ketoacid Dehydrogenase,Branched-Chain 2-Oxo Acid Dehydrogenase,Branched-Chain Keto Acid Dehydrogenase,Branched-Chain Oxo-Acid Dehydrogenase,2 Oxoisocaproate Dehydrogenase,Acid Dehydrogenase, Alpha-Keto,Alpha Keto Acid Dehydrogenase,Branched Chain 2 Oxo Acid Dehydrogenase,Branched Chain Alpha Keto Acid Decarboxylase,Branched Chain Keto Acid Dehydrogenase,Branched Chain Oxo Acid Dehydrogenase,Decarboxylase, BCKA,Dehydrogenase, 2-Oxoisocaproate,Dehydrogenase, Alpha-Keto Acid,Dehydrogenase, Branched-Chain Oxo-Acid,Oxo-Acid Dehydrogenase, Branched-Chain
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus