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In an attempt to obtain certain sub-surface spermatozoa antigens, human spermatozoa were treated with N-cetylpyridinium chloride in basic buffer, The extract was further ultrasonated and separated with gel chromatography over a Sephadex--G--100 column. The obtained fractions were lyophilized and tested against human sperm agglutinating and sperm immobilizing sera, in order to detect their antigenicity. Positive and negative controls revealed intensive precipitations and no precipitations respectively. Fraction A4 reacted intensively with sperm immobilizing sera. The final objectives of these sperm antigens solubilization studies are twofold. On the one hand we are looking for a method to lower sperm antibodies titres in sterile females, and on the other hand we are seeking an immunological contraceptive method.
L Mettler, and
N Steinhardt-Degola, and
H Skrabei
January 1977,
European journal of obstetrics, gynecology, and reproductive biology,
L Mettler, and
N Steinhardt-Degola, and
H Skrabei
January 1982,
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan,
L Mettler, and
N Steinhardt-Degola, and
H Skrabei
June 1979,
Nihon Hinyokika Gakkai zasshi. The japanese journal of urology,
L Mettler, and
N Steinhardt-Degola, and
H Skrabei
December 1978,
Journal de gynecologie, obstetrique et biologie de la reproduction,
L Mettler, and
N Steinhardt-Degola, and
H Skrabei
June 1981,
Nihon Hinyokika Gakkai zasshi. The japanese journal of urology,
L Mettler, and
N Steinhardt-Degola, and
H Skrabei
January 1979,
Zentralblatt fur Gynakologie,
L Mettler, and
N Steinhardt-Degola, and
H Skrabei
December 1979,
Radioisotopes,
L Mettler, and
N Steinhardt-Degola, and
H Skrabei
May 1978,
Casopis lekaru ceskych,
L Mettler, and
N Steinhardt-Degola, and
H Skrabei
January 1980,
Zentralblatt fur Gynakologie,
L Mettler, and
N Steinhardt-Degola, and
H Skrabei
June 1975,
Tijdschrift voor diergeneeskunde,
