Biomaterial Database

In vitro formation of glucose 6-phosphate from 3-phosphoglycerate by rat liver cytosol. 1981

S Mörikofer-Zwez, and F B Stoecklin, and P Walter

Liver cytosol preparations from 48 h starved rats, supplemented with 3 mM ATP, 0.5 mM sn-glycerol 3-phosphate, 10mM MgSO4 and a NADH-regenerating system showed a net glucose 6-phosphate formation from 2mM 3-phosphoglycerate of 0.72 micromol x min-1 x (g liver)-1. Glycerol 3-phosphate slightly increased when added at 0.5 mM; in its absence, dihydroxyacetone-phosphate formed from 3-phosphoglycerate was mainly reduced to glycerol 3-phosphate at the expense of fructose 1,6-bisphosphate and glucose 6-phosphate synthesis; when glycerol 3-phosphate was added at higher concentrations, net utilization occurred. The rate of glucose 6-phosphate formation positively correlated with the concentration of 3-phosphoglycerate and with the ratio of ATP/ADP. Increasing NADH concentrations stimulated the flow from 3-phosphoglycerate to glyceraldehyde 3-phosphate but also enhanced the reduction of dihydroxyacetone phosphate to glycerol 3-phosphate; as a result, the concentration of free NADH had little effect on the rate of glucose 6-phosphate formation. Lowering the concentration of magnesium from 10mM to 4mM, corresponding to free Mg2 concentrations of 7 and 0.6 mM respectively, resulted in a 30% increase of the rate of glucose 6-phosphate formation. The physiological relevance of the results presented is discussed.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D007773	Lactates	Salts or esters of LACTIC ACID containing the general formula CH3CHOHCOOR.
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008274	Magnesium	A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
D008297	Male		Males
D009243	NAD	A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)	Coenzyme I,DPN,Diphosphopyridine Nucleotide,Nadide,Nicotinamide-Adenine Dinucleotide,Dihydronicotinamide Adenine Dinucleotide,NADH,Adenine Dinucleotide, Dihydronicotinamide,Dinucleotide, Dihydronicotinamide Adenine,Dinucleotide, Nicotinamide-Adenine,Nicotinamide Adenine Dinucleotide,Nucleotide, Diphosphopyridine
D003600	Cytosol	Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.	Cytosols
D005958	Glucosephosphates
D005994	Glycerophosphates	Any salt or ester of glycerophosphoric acid.	Glycerolphosphate,Glycerophosphate,Calcium Glycerophosphate,Glycerolphosphates,Glycerophosphate, Calcium
D000244	Adenosine Diphosphate	Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.	ADP,Adenosine Pyrophosphate,Magnesium ADP,MgADP,Adenosine 5'-Pyrophosphate,5'-Pyrophosphate, Adenosine,ADP, Magnesium,Adenosine 5' Pyrophosphate,Diphosphate, Adenosine,Pyrophosphate, Adenosine