Biomaterial Database

Role of outer envelope contamination in protection elicited by ribosomal preparations against Neisseria gonorrhoeae infection. 1981

M D Cooper, and M J Wannemuehler, and R D Miller, and M F Fedyk

A recent report (Cooper et al., Infect. Immun. 28:92-100, 1980) demonstrated that immunization of guinea pigs with ribosomal preparations was protective (approximately 90%) against chamber infections with Neisseria gonorrhoeae. Similar protection has been demonstrated with other cellular immunogens such as outer membranes (OM) (92%) or purified lipopolysaccharide (LPS) (83%). Protection of LPS (5 to 100 micrograms) was dose dependent (83% with 100 micrograms). Treatment of LPS with pronase reduced the protection by 50%. Ribosomal preparations contained LPS contamination (3.9%) based on dry weight determinations by 2-keto-3-deoxyoctonate analysis. Analysis of ribosomal preparations isolated from cells after lactoperoxidase-mediated 125I labeling indicated a major OM contamination (Protein I). The ribosomal preparation also contained low levels of succinic and lactic dehydrogenase. Passive hemagglutination tests revealed that sera from guinea pigs immunized with ribosomal preparations also demonstrated antibody to OM proteins and LPS. LPS was able to absorb one line of precipitation seen in immunodiffusion reactions as well as the bactericidal activity of such sera. OM preparations were unable to absorb the remaining precipitin line or remove the bactericidal activity. It appears that LPS is the major antigen responsible for the bactericidal activity seen in ribosome-immune sera.

UI	MeSH Term	Description	Entries
D007106	Immune Sera	Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.	Antisera,Immune Serums,Sera, Immune,Serums, Immune
D008070	Lipopolysaccharides	Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)	Lipopolysaccharide,Lipoglycans
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D001770	Blood Bactericidal Activity	The natural bactericidal property of BLOOD due to normally occurring antibacterial substances such as beta lysin, leukin, etc. This activity needs to be distinguished from the bactericidal activity contained in a patient's serum as a result of antimicrobial therapy, which is measured by a SERUM BACTERICIDAL TEST.	Activities, Blood Bactericidal,Activity, Blood Bactericidal,Bactericidal Activities, Blood,Bactericidal Activity, Blood,Blood Bactericidal Activities
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D005260	Female		Females
D006069	Gonorrhea	Acute infectious disease characterized by primary invasion of the urogenital tract. The etiologic agent, NEISSERIA GONORRHOEAE, was isolated by Neisser in 1879.	Neisseria gonorrhoeae Infection
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000042	Absorption	The physical or physiological processes by which substances, tissue, cells, etc. take up or take in other substances or energy.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia