Biomaterial Database

Separation of 5-alkyluracils and purine bases in hydrolysates of enzymatically synthesized nucleic acids by high-performance ion-pair liquid chromatography. 1981

A H Csárnyi, and M Vajda, and J Sági

Reversed-phase and reversed-phase ion-pair chromatographic methods were used to determine 5-alkyluracils in the presence of purine bases (mainly adenine) in the hydrolysates of enzymatically synthesized DNA. The effect of ionic strength, pH, concentration of the ion-pairing agent and methanol on the selectivity between alkyluracils and purine bases was examined in order to simplify the routine work and to reduce the time necessary for analysis. Optimal conditions could be developed for the isocratic separation of the various mixtures obtained by hydrolysis of the products of enzymatic synthesis.

UI	MeSH Term	Description	Entries
D009696	Nucleic Acids	High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.	Nucleic Acid,Acid, Nucleic,Acids, Nucleic
D011687	Purines	A series of heterocyclic compounds that are variously substituted in nature and are known also as purine bases. They include ADENINE and GUANINE, constituents of nucleic acids, as well as many alkaloids such as CAFFEINE and THEOPHYLLINE. Uric acid is the metabolic end product of purine metabolism.
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000432	Methanol	A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness.	Alcohol, Methyl,Carbinol,Sodium Methoxide,Wood Alcohol,Alcohol, Wood,Methoxide, Sodium,Methyl Alcohol
D014498	Uracil	One of four nucleotide bases in the nucleic acid RNA.