Biomaterial Database

[Properties of the obligate methylotroph Methylophilus methanolovorus]. 1981

N V Loginova, and Iu A Trotsenko

A strain of obligate methylotrophic bacteria utilizing methanol as a sole source of carbon and energy was isolated from the activated sludge. The bacteria are strictly aerobic Gram-negative non-pigmented motile rods with a single polar flagellum. They do not form spores or capsules and have no complex intracytoplasmic membranes. They do not require vitamins and assimilate methanol via the hexulose phosphate pathway. The G+C content of DNA is 51.0 +/- 0.5 mole%. The optimal temperature is 30-38 degrees C, the optimal pH is 6.8-7.4. The obligate dependence on C1-substrates but not on methane, as well as the absence of complex intracytoplasmic membranes, makes it possible to assign such methylotrophs to the genus Methylophilus nov. gen. The description of the new genus and species M. methanolovorus nov. gen., nov. sp. is presented.

UI	MeSH Term	Description	Entries
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D003596	Cytosine	A pyrimidine base that is a fundamental unit of nucleic acids.
D004269	DNA, Bacterial	Deoxyribonucleic acid that makes up the genetic material of bacteria.	Bacterial DNA
D005407	Flagella	A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)	Flagellum
D006088	Gram-Negative Aerobic Bacteria	A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.	Achromatiaceae,Achromatium,Achromobacteriaceae,Gram Negative Aerobic Bacteria
D006147	Guanine
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000432	Methanol	A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness.	Alcohol, Methyl,Carbinol,Sodium Methoxide,Wood Alcohol,Alcohol, Wood,Methoxide, Sodium,Methyl Alcohol
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures